论文部分内容阅读
观察１－（２，６－二甲基苯氧基）－２－（３，４－二甲氧基苯乙胺基）丙烷盐酸盐（ＤＤＰＨ）对实验性腹主动脉狭窄高血压大鼠血管平滑肌细胞（ＶＳＭＣ）增殖的作用及对原癌基因及抑癌基因的影响。方法氚－胸腺嘧啶核苷（３Ｈ－ＴｄＲ）掺入，电镜，原位杂交及Ｎｏｒｔｈｅｒｎｂｌｏｔ杂交。结果ＤＤＰＨ在降低血压同时，能减少血管平滑肌细胞（ＶＳＭＣ）的线粒体，粗面内质网及３Ｈ－ＴｄＲ掺入量（Ｐ＜０．０１），并能逆转ｃ－ｆｏｓ，ｃ－ｍｙｃ，ｃ－ｓｉｓ原癌基因ｍＲＮＡ表达增强（Ｐ＜０．０５～０．０１），Ｐ５３抑癌基因ｍＲＮＡ表达减弱（Ｐ＜０．０５）。结论ＤＤＰＨ能抑制实验性高血压大鼠的ＶＳＭＣ增殖，与癌基因调控的分子生物学机制有关。
To observe the effects of 1- (2,6-dimethylphenoxy) -2- (3,4-dimethoxyphenethyl) propane hydrochloride (DDPH) on experimental abdominal aortic stenosis in hypertensive rats Proliferation of vascular smooth muscle cells (VSMCs) and its effects on proto-oncogenes and tumor suppressor genes. Methods Tritiated-thymidine incorporation, electron microscopy, in situ hybridization and Northern blot hybridization. Results DDPH decreased the blood pressure and decreased the incorporation of mitochondria, rough endoplasmic reticulum and 3H-TdR in vascular smooth muscle cells (VSMC) (P <0.01), and reversed the expression of c-fos, c-myc and c -sis proto-oncogene mRNA expression (P <0.05 ~ 0.01), P53 tumor suppressor gene mRNA expression decreased (P <0.05). Conclusion DDPH can inhibit VSMC proliferation in experimental hypertensive rats, which is related to the molecular biological mechanism of oncogene regulation.