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目的观察miRNA-29c(miR-29c)对前列腺癌细胞系LNCaP增殖和侵袭能力的影响,探讨其潜在的应用价值。方法采用qRT-PCR观察雄激素依赖性(LNCaP)与雄激素非依赖性(LNCaP-AI)前列腺癌细胞中miR-29c的表达差异;采用miR-29c抑制物(anti-miR-29c)下调LNCaP细胞中miR-29c的表达量,绘制LNCaP细胞生长曲线,利用流式细胞仪测定细胞周期;Transwell侵袭实验分析miR-29c抑制前后LNCaP细胞的体外侵袭能力。结果 qRT-PCR显示LNCaP-AI细胞中miR-29c水平明显低于LNCaP细胞(P<0.05);下调LNCaP细胞中miR-29c的表达量,可明显促进LNCaP细胞体外增殖及侵袭能力(P<0.05)。结论 miR-29c的正常表达有利于抑制LNCaP细胞的体外增殖和侵袭,有望成为前列腺癌生物治疗的潜在靶点。
Objective To observe the effect of miRNA-29c (miR-29c) on the proliferation and invasion of prostate cancer cell line LNCaP and to explore its potential value. Methods The expression of miR-29c in androgen-dependent (LNCaP-AI) prostate cancer cells was detected by qRT-PCR. The expression of LNCaP was down-regulated by anti-miR-29c The expression of miR-29c in the cells was measured. The growth curve of LNCaP cells was drawn and the cell cycle was determined by flow cytometry. The invasion ability of LNCaP cells in vitro and in vivo was analyzed by Transwell invasion assay. Results qRT-PCR showed that the level of miR-29c in LNCaP-AI cells was significantly lower than that in LNCaP cells (P <0.05). The down-regulation of miR-29c expression in LNCaP cells significantly promoted the proliferation and invasion ability of LNCaP cells in vitro ). Conclusion The normal expression of miR-29c is helpful to inhibit the proliferation and invasion of LNCaP cells in vitro, which is expected to be a potential target for bioprocessing of prostate cancer.