采用液氮冷冻Ｗｉｓｔａｒ大鼠一侧大脑制成血管源性脑水肿模型，将大鼠全脑沿冷冻中心制成冠状面冰冻切片，运用免疫组化染色观察脑水肿组及对照组白质血脑屏障内皮细胞膜表面ＩＣＡＭ１蛋白表达量的变化，并对此进行图像分析，发现大鼠内皮细胞膜在正常情况下表达少量的ＩＣＡＭ１蛋白分子，梗塞后３ｈ有明显的升高（Ｐ＜０００１），６～１２ｈ达高峰，以后逐渐恢复，至２４ｈ后基本降至冷冻前表达量水平。ＩＣＡＭ１蛋白表达量的高峰时间与脑水肿达高峰的时间一致 The model of vasogenic brain edema was made by freezing the brain of one side of liquid nitrogen in Wistar rats. The whole brain of rat was made coronal frozen section along the freezing center. The white matter blood-brain barrier of cerebral edema group and control group were observed by immunohistochemistry The change of ICAM1 protein expression on endothelial cell membrane surface, and image analysis, found that under normal conditions, the rat endothelial cell membrane expressed a small amount of ICAM1 protein molecule, which was significantly increased at 3h after infarction (P <0 001), peaked at 6 ~ 12h, then gradually recovered, and basically dropped to the level before freezing after 24 hours. ICAM 1 protein expression peak time and brain edema reached the peak time consistent