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目的研究伤寒沙门菌质粒pRST98对生物膜形成的影响。方法将携带pRST98的野生型伤寒沙门菌于30℃和37℃培养3 d,用半定量法确定生物膜形成的适宜温度。将稀释的菌液于30℃分别培养12 h、1 d、2 d、3 d、4 d和5 d,用半定量法确定生物膜成熟的时间。分别用携带pRST98的野生型伤寒沙门菌,消除pRST98的突变株及pRST98的回补株建立生物膜模型,用结晶紫染色法,半定量法和扫描电镜观察三种株受试菌形成生物膜的能力。结果在30℃培养时细菌生物膜形成能力高于37℃,此温度为沙门菌生物膜形成的适宜温度;3 d时生物膜趋于成熟;野生株和回补株形成生物膜的能力显著高于突变株。结论伤寒沙门菌质粒pRST98与该菌生物膜形成密切相关。
Objective To study the effect of Salmonella typhi plasmid pRST98 on biofilm formation. Methods Salmonella typhimurium harboring pRST98 was cultured at 30 ℃ and 37 ℃ for 3 days, and the suitable biofilm formation temperature was determined by semi-quantitative method. The diluted bacterial broths were cultured at 30 ℃ for 12 h, 1 d, 2 d, 3 d, 4 d and 5 d, respectively, and the biomembrane maturation time was determined by semi-quantitative method. The biofilm model was constructed by using wild-type Salmonella typhimurium harboring pRST98, eliminating the mutants of pRST98 and the complement of pRST98, respectively. The biofilm formation was observed by crystal violet staining, semi-quantitative and scanning electron microscopy ability. Results The bacterial biofilm formation ability was higher than 37 ℃ when cultured at 30 ℃, which was the optimum temperature for the formation of Salmonella biofilm. The biofilm tended to mature on the 3rd day, and the biofilm formation ability of the wild strain and the retrofit strain was significantly higher In mutant strains. Conclusion Salmonella typhi plasmid pRST98 is closely related to the biofilm formation.