Objective: The aim of this study was to investigate the effect of toremifene on A549 human lung adenocarcinoma cells, and its sensibilization with gemcitabine, so that to provide a new clinical approach for non-small-cell lung cancer(NSCLC). Methods: A549 cells were seeded into 96-well plates and exposed to different agents(gemcitabine or gemcitabine with toremifene). The cytotoxicity of each agent was evaluated by MTT, cell cycle and apoptotic rate were detected by flow cytometry(FCM). Results: 1. By using FCM, we found A549 cells in S and G2/M phases with toremifene decreased but increased in G0/G1 phase. The higher concentration of toremifene, the more decreased was when compared with the control group. 2. FCM showed toremifene’s apoptosis effect on A549 cells increased with its increasing dose. 3. By MTT, toremifene had no cytotoxic effect on A549 cells at the concentration of 5 or 2.5 μmol/L. The IC50 of gemcitabine to A549 was 34.51 μmol/L, and the combined group was 13.59 μmol/L. Conclusion: Toremifene could inhibit the growth of A549 human lung adenocarcinoma cells. Toremifene combined with gemcitabine showed significantly remarkable chemotherapy sensibilization on A549 human lung adenocarcinoma cells. Objective: The aim of this study was to investigate the effect of toremifene on A549 human lung adenocarcinoma cells, and its sensibilization with gemcitabine, so that to provide a new clinical approach for non-small-cell lung cancer (NSCLC). Methods: A549 The cells were seeded into 96-well plates and exposed to different agents (gemcitabine or gemcitabine with toremifene). The cytotoxicity of each agent was evaluated by MTT, cell cycle and apoptotic rate were detected by flow cytometry (FCM). using FCM, we found A549 cells in S and G2 / M phases with toremifene decreased but increased in G0 / G1 phase. The higher concentration of toremifene, the more decreased was when compared with the control group. 2. FCM showed toremifene’s apoptosis effect on The IC50 of gemcitabine to A549 was 34.51 μmol / L, and the combined group was 13.59 μmo l / L. Conclusion: Toremifene could inhibit the growth of A549 human lung adenocarcinoma cells. Toremifene combined with gemcitabine showed significant prednisone sensibilization on A549 human lung adenocarcinoma cells.