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目的:研究茶叶多糖对小鼠骨髓造血细胞表面抗原表达以及脾淋巴细胞增殖的影响。方法:应用纳米生物技术、免疫学技术和现代细胞生物学研究技术观察了靶细胞表面分化抗原和小鼠脾淋巴细胞的增殖活性。结果:FACS检测显示茶多糖对CD4+CD19+CD119+的表达均有促进作用,CD34+细胞随培养时间的延长逐渐减少,各组数值与对照组比,均有明显差异(p<0.01)。MTT结果显示,茶多糖诱导的各组小鼠脾淋巴细胞OD值也明显上升(P<0.01)。结论:茶多糖可促进骨髓造血干细胞向祖细胞分化并促进淋巴细胞的增殖,显示了对造血和免疫的正调节作用。此结果将为进一步开发茶叶的保健功能和治疗作用提供理论依据。
OBJECTIVE: To study the effect of tea polysaccharide on the expression of bone marrow hematopoietic cell surface antigen and the proliferation of splenic lymphocytes. Methods: The proliferation activity of target cell surface differentiation antigen and mouse splenic lymphocytes were observed using nano-biotechnology, immunological techniques and modern cell biology research techniques. RESULTS: FACS analysis showed that tea polysaccharides promoted the expression of CD4+CD19+CD119+, and CD34+ cells gradually decreased with the prolongation of culture time. There were significant differences between the values of each group and the control group (p<0.01). MTT results showed that the OD value of splenic lymphocytes in mice induced by tea polysaccharides also increased significantly (P<0.01). CONCLUSION: Tea polysaccharides can promote the differentiation of bone marrow hematopoietic stem cells to progenitor cells and promote the proliferation of lymphocytes, indicating a positive regulation of hematopoiesis and immunity. This result will provide a theoretical basis for the further development of the health functions and therapeutic effects of tea.