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Objective:To determine phytochemistry and larvicidal activity of Eucalyptus eamaldulensis against Anopheles stephensi.Methods:The chemical compositions of the leaf essential oils were analyzed using gas chromatography/mass spectrometry(GC/MS).The larvicidal activity of essential oils and extract of leaf were tested against 4th instar larvae of laboratory-reared Anopheles stephensi.Results:GC/MS analyses identified the presence of 28 compounds corresponding to 99.60%of the total oil.The main constituents in the leaf essential oil were 1,8-cineole(69.46%),γ-Terpinene(15.10%),α-Pinene(5.47%) and Globulol(2%).The leaf extract and volatile oil exerted significant larvicidal activity with LC_(50) values of 89.85 and 397.75 ppm,respectively.Clear dose-response relationships were established with the highest dose of 320 ppm essential oil extract resulted almost 100%mortality in the population.Conclusions:The larvicidal properties suggest that the essential oil of plant is a potential source of valuable larvicidal compounds against malaria vector and can be used as an alternative to synthetic insecticides.
Objective: To determine phytochemistry and larvicidal activity of Eucalyptus eamaldulensis against Anopheles stephensi. Methods: The chemical compositions of the leaf essential oils were analyzed using gas chromatography / mass spectrometry (GC / MS). The larvicidal activity of essential oils and extract of leaf were tested against 4th instar larvae of laboratory-reared Anopheles stephensi. Results: GC / MS analyzes identified the presence of 28 compounds corresponding to 99.60% of the total oil. The main constituents in the leaf essential oil were 1,8-cineole (69.46% The leaf extract and volatile oil exerted significant larvicidal activity with LC_ (50) values of 89.85 and 397.75 ppm, respectively.Clear dose (γ -Therpinene 15.10%), α-Pinene (5.47%) and Globulol (2% -response relationships were established with the highest dose of 320 ppm essential oil extract derived almost 100% mortality in the population. Conclusions: The larvicidal properties suggest that the essential oil of plant is a potential source of valuable larvicidal compounds against malaria vector and can be used as an alternative to synthetic insecticides.