论文部分内容阅读
目的检测手足口病患儿外周血淋巴细胞亚群,探讨其免疫功能变化及临床意义。方法收集手足口病患儿73例,根据病情分为普通组49例、重症组24例。以8例健康儿童作为对照,采用流式细胞术,检测73例手足口病患儿外周抗凝全血的淋巴细胞细胞亚群:CD3+细胞、CD4+细胞、CD8+细胞、CD19+细胞、CD16+56+细胞的相对计数。结果手足口病患儿重症重型组外周血CD3+细胞、CD4+细胞(Th)的百分率较对照组及普通组显著降低(P<0.01),CD8+细胞(Ts)的百分率亦低于普通组(P<0.05),而两组间Th/Ts细胞比值的变化不存在明显差异(P>0.05);CD19+细胞、CD16+56+细胞的百分率变化无明显差异(P>0.05)。结论手足口病患儿存在细胞免疫功能紊乱,淋巴细胞的变化以CD3+细胞、CD4+细胞为主,其在抗病毒感染中起着重要作用,可能参与了手足口病病情的发展。
Objective To detect peripheral blood lymphocyte subsets in children with hand-foot-mouth disease and to explore the changes of its immune function and its clinical significance. Methods 73 cases of hand-foot-mouth disease were collected, divided into general group (49 cases) and severe group (24 cases). The peripheral blood anticoagulant whole blood lymphocyte subsets of 73 children with HFMD were detected by flow cytometry in 8 healthy children: CD3 + cells, CD4 + cells, CD8 + cells, CD19 + cells, CD16 + 56 + The relative count of cells. Results The percentages of CD3 + cells and CD4 + cells (Th) in HFMD group were significantly lower than those in control group and normal group (P <0.01), and the percentage of CD8 + cells (Ts) 0.05). There was no significant difference in Th / Ts ratio between the two groups (P> 0.05). There was no significant difference in the percentage of CD16 + 56 + cells (P> 0.05). Conclusions There are cellular immune dysfunction in children with HFMD. The changes of lymphocytes are mainly CD3 + cells and CD4 + cells, which plays an important role in antiviral infection and may be involved in the development of HFMD.