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目的 :观察紫杉醇对人卵巢癌细胞系A2 780凋亡的作用 ,探讨其对卵巢癌细胞survivin基因表达的影响。方法 :应用MTT、流式细胞仪等方法研究紫杉醇对卵巢癌细胞系A2 780凋亡的诱导作用 ,运用RT- PCR分析紫杉醇对卵巢癌细胞survivin表达的影响。结果 :紫杉醇可诱导卵巢癌细胞系A2 780凋亡 ,不同浓度的紫杉醇引起的凋亡率不同 ,在紫杉醇浓度≥ 10 - 7mol.L- 1时 ,凋亡率显著升高。经紫杉醇作用后的卵巢癌细胞表达sur vivin较用药前明显下降。结论 :紫杉醇可能通过抑制survivin的表达 ,促进肿瘤细胞凋亡。
OBJECTIVE: To observe the effect of paclitaxel on the apoptosis of human ovarian cancer cell line A2 780 and to explore its effect on the expression of survivin gene in ovarian cancer cells. Methods: The apoptosis of ovarian cancer cell line A2 780 induced by paclitaxel was studied by MTT and flow cytometry. The effect of paclitaxel on the expression of survivin in ovarian cancer cell line was analyzed by RT-PCR. Results: Paclitaxel induced the apoptosis of ovarian cancer cell line A2 780. The apoptotic rates of paclitaxel induced by different concentrations of paclitaxel were different. When paclitaxel concentration was 10 - 7 mol.L - 1, the apoptosis rate was significantly increased. Survivin expression in ovarian cancer cells after paclitaxel treatment was significantly lower than that before treatment. Conclusion: Paclitaxel may promote tumor cell apoptosis by inhibiting the expression of survivin.