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目的：为准确、迅速诊断乙型肝炎，本研究在１１３例母婴血清标本中建立套式ＰＣＲ检测技术并与ＰＣＲ方法进行比较。方法：应用套式聚合酶链（ＰＣＲ）法检测ＨＢＶ－ＤＮＡ１１３例。结果：１１３例孕妇，第一对引物ＰＣＲ扩增１０１例阳性，第二对引物ＰＣＲ扩增９７例阳性。７７例为乙肝的婴儿，第一对引物ＰＣＲ扩增６９例阳性，第二对引物ＰＣＲ扩增６５例阳性。二对引物经套式ＰＣＲ，孕妇又有１２例阳性，其中阳性率提高８．８５％，婴儿有８例阳性，阳性率提高７．８％。结论：套式ＰＣＲ比ＰＣＲ方法更快、更敏感、更特异、更简便，直接支持了母婴垂直感染的论点
Objective: To accurately and rapidly diagnose hepatitis B, a nested PCR assay was developed in 113 maternal and infant serum samples and compared with PCR. Methods: 113 cases of HBV-DNA were detected by nested polymerase chain reaction (PCR). Results: 113 pregnant women, the first pair of primers PCR amplification of 101 cases of positive, the second pair of primers PCR amplification of 97 cases were positive. Of the 77 infants with hepatitis B, 69 were positive for PCR amplification of the first pair of primers and 65 were positive for PCR amplification of the second pair of primers. Two sets of primers by nested PCR, pregnant women and 12 cases were positive, of which the positive rate increased 8.85%, 8 infants were positive, the positive rate increased 7.8%. Conclusion: Nested PCR is faster, more sensitive, more specific and easier than PCR, which directly supports the argument of vertical infection in mother and baby