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目的:为准确、迅速诊断乙型肝炎,本研究在113例母婴血清标本中建立套式PCR检测技术并与PCR方法进行比较。方法:应用套式聚合酶链(PCR)法检测HBV-DNA113例。结果:113例孕妇,第一对引物PCR扩增101例阳性,第二对引物PCR扩增97例阳性。77例为乙肝的婴儿,第一对引物PCR扩增69例阳性,第二对引物PCR扩增65例阳性。二对引物经套式PCR,孕妇又有12例阳性,其中阳性率提高8.85%,婴儿有8例阳性,阳性率提高7.8%。结论:套式PCR比PCR方法更快、更敏感、更特异、更简便,直接支持了母婴垂直感染的论点
Objective: To accurately and rapidly diagnose hepatitis B, a nested PCR assay was developed in 113 maternal and infant serum samples and compared with PCR. Methods: 113 cases of HBV-DNA were detected by nested polymerase chain reaction (PCR). Results: 113 pregnant women, the first pair of primers PCR amplification of 101 cases of positive, the second pair of primers PCR amplification of 97 cases were positive. Of the 77 infants with hepatitis B, 69 were positive for PCR amplification of the first pair of primers and 65 were positive for PCR amplification of the second pair of primers. Two sets of primers by nested PCR, pregnant women and 12 cases were positive, of which the positive rate increased 8.85%, 8 infants were positive, the positive rate increased 7.8%. Conclusion: Nested PCR is faster, more sensitive, more specific and easier than PCR, which directly supports the argument of vertical infection in mother and baby