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在石林和寻甸两个不同气候生态区通过田间试验研究了烤烟不同施氮量与打顶时(即赤星病发病初期)健康烟株烟叶中的含氮量以及类黄酮、总酚、可溶性糖和游离性氨基酸含量等部分生理生化指标以及后期赤星病发生三者间的相互关系。结果表明,尽管两个试验点烟株体内的生理生化状况和赤星病发生程度有一定的差异,但施氮量与上述生理生化指标、赤星病发生三者间的关系都有一致的规律,即:随着施氮量增加,烟叶内游离性氨基酸含量和氮累积量增加,类黄酮、总酚和可溶性糖含量下降;烟叶成熟过程中不同时间赤星病发病情况均随施氮量增加而增加,其中,高氮和不施氮处理间差异显著。相关分析表明,烟株赤星病发病初期、中期和后期的发病率和病情指数均与发病初期烟叶内的类黄酮、总酚和可溶性糖含量有显著负相关关系;而与游离性氨基酸含量和氮累积量有显著正相关关系。这表明不同施氮量烟株成熟期赤星病发生的差异在一定程度上与发病初期烟叶中的类黄酮、总酚、可溶性糖和游离性氨基酸含量以及氮累积量的差异有关;同时还表明通过调控施氮量可以较好地控制赤星病病害的发生。
A field experiment was conducted in two different climate ecotopes of Shilin and Xundian to study the effects of different nitrogen application rates and tops (the initial stage of the onset of erythematosus disease) on the nitrogen contents of tobacco leaves, flavonoids, total phenols, soluble sugars And free amino acid content of some physiological and biochemical indicators and the occurrence of later brown spot disease between the three interrelationship. The results showed that although there was a certain difference in physiological and biochemical status between the two experimental tobacco plants and the occurrence of brown spot disease, the relationship between the nitrogen application rate and the above physiological and biochemical indexes and the occurrence of brown spot disease had the same rule : With the increase of nitrogen application rate, the content of free amino acids and nitrogen in tobacco leaves increased, while the content of flavonoids, total phenols and soluble sugar decreased. The incidence of brown plant disease at different time during tobacco maturation increased with the increase of nitrogen application rate, Among them, there was significant difference between high nitrogen and non-nitrogen treatment. Correlation analysis showed that there was a significant negative correlation between the incidence and the disease index of early stage, middle stage and later stage of brown plant disease and the contents of flavonoids, total phenols and soluble sugars in the early stage tobacco leaves. However, the correlations between free amino acid content and nitrogen Cumulative significant positive correlation. This indicates that the difference of the occurrence of brown spot disease at maturity stage of different nitrogen application rates is related to the differences of flavonoids, total phenols, soluble sugar and free amino acid content and nitrogen accumulation in the tobacco leaves at the early stage of the disease. At the same time, Regulation of nitrogen application can control the occurrence of brown spot disease.