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Bombyx mori nuclear polyhedrosis virus (BmNPV) DNA has been cleaved with restriction endonuclease SalI into 29 fragments of different sizes. The range of the molecular weight of these fragments as detected by electrophoresis on agarose gel is from 0.70 Kb to 10.0Kb. 24 SalI fragments were cloned in plasmid pBR322. The sum of the molecular weight of cloned BmNPV DNA fragments accounts for about 80% of the virus genome DNA.
Bombyx mori nuclear polyhedrosis virus (BmNPV) DNA has been cleaved with restriction endonuclease SalI into 29 fragments of different sizes. The range of the molecular weight of these fragments as detected by electrophoresis on agarose gel is from 0.70 Kb to 10.0 Kb. 24 SalI fragments were cloned in plasmid pBR322. The sum of the molecular weight of cloned BmNPV DNA fragments accounts for about 80% of the virus genome DNA.