目的评价应用新型国产试剂进行微剂量（３７ＫＰｑ）１４Ｃ－尿素呼气试验（１４Ｃ－ＵＢＴ）对幽门螺杆菌（ＨＰ）感染的诊断价值。方法５９例ＨＰ阳性病人和４７例ＨＰ阴性对照接受试验。试验所用试剂全部国产化，包括１４Ｃ－尿素、ＣＯ２吸收剂、闪烁剂。ＲＯＣ分析法确定１４Ｃ－ＵＢＴ最适判别值。１９例志愿者第二日重复试验。结果ＲＯＣ分析法得出１４Ｃ－ＵＢＴ最适判别值为２５０ｄｐｍ／ｍｍｏｌＣＯ２。实验对ＨＰ感染诊断的敏感性为９６．６％（５７／５９），特异性９５．７％（４５／４７），准确性９６．２％（１０２／１０６），实验重复性良好。体重校正和使用液闪仪本底计算测试结果不影响诊断效能。结论国产试剂微剂量１４Ｃ－ＵＢＴ诊断ＨＰ感染具有高度的准确性，且简单和安全，试验无需试餐和加用非标记尿素，不需收集呼气本底，结果不需以体重校正 Objective To evaluate the diagnostic value of 14C-urea breath test (14C-UBT) in the diagnosis of Helicobacter pylori (HP) infection using a novel domestic reagent. Methods 59 cases of HP-positive patients and 47 cases of HP-negative control were tested. All domestic reagents used in the experiment, including 14C-urea, CO2 absorbers, scintillators. ROC analysis to determine the optimal 14C-UBT discriminant value. Nineteen volunteers repeated the test on the second day. Results ROC analysis obtained 14C-UBT optimum discrimination value of 250dpm / mmolCO2. The sensitivity of the experiment to the diagnosis of HP infection was 96.6% (57/59), the specificity was 95.7% (45/47) and the accuracy was 96.2% (102/106). The experiment had good repeatability. Body weight correction and the use of a liquid-flash instrument to calculate the test results do not affect the diagnostic performance. Conclusion The domestic reagent micro-dose 14C-UBT diagnosis of HP infection with a high degree of accuracy, and simple and safe, no test meal and add non-labeled urea, without the acquisition of expired background, the results do not need to be corrected for body weight