论文部分内容阅读
目的用基因敲入Ca V1.1-R528H小鼠建立甲亢性低钾型周期性麻痹模型并对其进行评价。方法8周龄基因敲入Ca V1.1-R528H雄性小鼠及8周龄野生型C57BL/6J雄性小鼠各36只,采用三因素两水平2×2×2析因设计方法按体重随机原则(三因素分别为突变、甲状腺素及胰岛素因素,两水平为有或无)分为8组。其中有甲状腺素处理组的小鼠制备高甲状腺素毒症,按350μg/kg体重连续腹腔注射左旋甲状腺素钠12 d,末次给药后有胰岛素处理组按0.8 U/kg体重给予腹腔注射短效胰岛素,分别检测并记录各组小鼠注射前(0 min)及注射后(30、60 min)的血钾。结果 (1)制备高甲状腺素毒症的小鼠出现烦躁不安、易激怒及毛色枯燥现象,相比对照组,饮食及饮水量明显增多,而体重增加缓慢。甲状腺功能检测显示T3、T4明显高于相应对照组,TSH明显低于相应对照组,且差异均有显著性(P<0.05)。(2)单独给予甲状腺素或胰岛素处理,突变组与野生组血钾同时间点比较并没有统计学差异,而在高甲状腺素毒症下给予胰岛素处理后,突变组与野生组同时间点(30、60 min)比较突变组血钾显著低于野生组(P<0.05)。(3)主效应及交互作用:单独突变因素或甲状腺素因素对血钾并没有作用,仅有胰岛素对降低血钾有作用(P<0.05);甲状腺素因素和突变因素之间以及胰岛素因素和突变因素之间均有交互作用(P<0.05);甲状腺素因素和胰岛素因素之间没有交互作用。结论 (1)高甲状腺素毒症制备成功。(2)利用基因敲入Ca V1.1-R528H小鼠成功的建立了甲亢性低钾型周期性麻痹模型。
Objective To establish a hyperthyroid hypokalemic model of periodic paralysis using gene knock-in Ca V1.1-R528H mice and evaluate the results. Methods Eight-week-old male Ca V1.1-R528H knock-in mice and 36 male W8-week-old wild-type C57BL / 6J mice were randomly divided into two groups according to the principle of random weight (Three factors were mutation, thyroid hormone and insulin factors, with or without the two levels) into 8 groups. Thyroxine-treated mice were given hypothyroxine at a dose of 350 μg / kg body weight for 12 days. The mice in the insulin-treated group were treated with 0.8 U / kg of body weight and given short-acting intraperitoneal injection Insulin were detected and recorded in each group before injection of mice (0 min) and after injection (30,60 min) of serum potassium. Results (1) Hypothyroidism mice developed irritability, irritability and hair color boring phenomenon, compared with the control group, diet and water intake increased significantly, while weight gain slow. Thyroid function tests showed that T3, T4 were significantly higher than the corresponding control group, TSH was significantly lower than the corresponding control group, and the difference was significant (P <0.05). (2) Thyroxine or insulin treatment alone, mutation group and wild group at the same time point of blood potassium was no statistically significant difference, while in hyperthyroidism under insulin treatment, the mutant group and the wild group at the same time point ( 30,60 min), the serum potassium of the mutant group was significantly lower than that of the wild group (P <0.05). (3) The main effect and interaction: the mutation alone or thyroxine has no effect on the serum potassium, only the insulin has the effect of lowering the serum potassium (P <0.05); thyroxine and the mutation as well as the insulin and There was interaction between mutation factors (P <0.05); there was no interaction between thyroxine and insulin. Conclusion (1) High thyroxine poisoning was successfully prepared. (2) Hypokalemic hypokalemic model of periodic paralysis was successfully established by using gene knock-in Ca V1.1-R528H mice.