目的了解产ESBLs肺炎克雷伯菌的整合子存在状况。方法用PCR方法扩增I类整合酶基因,经电泳后检测扩增产物。结果72株产ESBLs肺炎克雷伯菌中检测出Ⅰ类整合子67株,检出率为93.0%,Ⅰ类整合子阳性菌对氨基糖苷类、喹诺酮类及头孢菌素类药物表现出较高的耐药,其多重耐药率明显高于I类整合子阴性菌株(P<0.05)。结论Ⅰ类整合子广泛地存在产ESBLs肺炎克雷伯菌中,Ⅰ类整合子对细菌多重耐药性的产生和传播起着重要作用。 Objective To understand the presence of integron in Klebsiella pneumoniae producing ESBLs. Methods The type I integrase gene was amplified by PCR and detected by electrophoresis. Results Of the 72 ESBLs-producing Klebsiella pneumoniae strains, 67 strains of type Ⅰ integron were detected, the detection rate was 93.0%. The positive strains of class Ⅰ integron showed high levels of aminoglycosides, quinolones and cephalosporins The multi-drug resistance rate was significantly higher than that of the class I integron-negative strains (P <0.05). Conclusion Class I integrons exist widely in ESBLs-producing Klebsiella pneumoniae. Class I integrons play an important role in the generation and transmission of bacterial multi-drug resistance.