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目的:建立芍桂胶囊的主要成分芍药苷与肉桂酸的高效液相色谱法含量测定的方法。方法:采用双波长双通道高效液相色谱法测定,色谱柱为大连依利特Hyper-siLODS2C18柱(46mm×250mm,5μm);流动相为0.1%醋酸-乙腈梯度洗脱,检测波长λ1=230nm,λ2=278nm。结果:芍药苷在28.8~172.8mg·L-1范围内线性关系良好,r=0.9982,平均回收率为90.15%(n=5);肉桂酸在3.85~46.27mg·L-1范围内线性关系良好,r=0.9988,平均回收率为89.63%(n=5)。结论:本法专属性强,操作简便,结果准确。适用于检测芍桂胶囊中芍药苷和肉桂酸的含量。
Objective: To establish a HPLC method for the determination of paeoniflorin and cinnamic acid, which are the main components of Citrus grandis capsules. Methods: The dual-wavelength dual-channel HPLC method was used to determine the column. The column was Dalian Hyper-SiLODS2C18 column (46mm×250mm, 5μm). The mobile phase was gradient elution with 0.1% acetic acid-acetonitrile. The detection wavelength was λ1=230nm. Λ2=278 nm. Results: There was good linearity in the range of 28.8~172.8 mg·L-1, with r=0.9982. The average recovery was 90.15% (n=5). The linear relationship of cinnamic acid was in the range of 3.85~46.27 mg·L-1. Good, r = 0.9988, with an average recovery of 89.63% (n = 5). Conclusion: This law is highly specific, simple and accurate. It is suitable for detecting the content of paeoniflorin and cinnamic acid in wolfberry capsules.