论文部分内容阅读
目的观察瘦素和瘦素拮抗剂对雄激素非依赖性前列腺癌细胞株DU-145增殖凋亡的影响。方法将100ng/ml重组人瘦素加入DU-145细胞体外培养24h后,分别加入不同浓度的(10、20、50、100、200ng/ml)重组人瘦素拮抗剂,继续培养24、48h。倒置显微镜观察细胞的形态变化,MTT法检测细胞抑制率,流式细胞仪检测细胞凋亡率。结果重组人瘦素作用下DU-145细胞贴壁生长旺盛,数目增多,染色质颗粒分布不均;重组人瘦素拮抗剂作用下DU-145细胞变小、变圆,细胞间隙明显,脱落漂浮。随着重组人瘦素拮抗剂药物浓度增加,DU-145细胞抑制率逐渐增加,与对照组比较差异有统计学意义(P<0.05),无时间依赖性。24h后50ng/ml瘦素拮抗剂组与对照组细胞凋亡率比较差异有统计学意义(P<0.05)。结论在体外培养条件下,瘦素拮抗剂能够抑制瘦素对DU-145细胞促增殖和抗凋亡作用,为雄激素非依赖性前列腺癌的治疗提供一种新的方法。
Objective To observe the effects of leptin and leptin antagonist on proliferation and apoptosis of androgen-independent prostate cancer cell line DU-145. Methods Human DU-145 cells were treated with 100ng / ml recombinant human leptin in vitro for 24 hours. Different concentrations of (10,20,50,100,200ng / ml) recombinant human leptin antagonist were added and cultured for 24,48 hours. The morphological changes of the cells were observed by inverted microscope. The inhibition rate of the cells was detected by MTT assay and the apoptosis rate by flow cytometry. RESULTS: DU-145 cells grew strongly adherent and increased in numbers, with uneven distribution of chromatin particles. The DU-145 cells became smaller and more rounded with clear intercellular leukocyte clearance and floating off . With the increase of concentration of recombinant human leptin antagonist, the inhibitory rate of DU-145 cells increased gradually, compared with the control group, the difference was statistically significant (P <0.05), no time-dependent. The apoptosis rate of 50 ng / ml leptin antagonist group and control group after 24h was significantly different (P <0.05). Conclusions Leptin antagonists can inhibit the proliferation and anti-apoptotic effects of leptin on DU-145 cells in vitro and provide a new method for the treatment of androgen-independent prostate cancer.