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目的:探讨生长相关性癌基因1(GRO1)在喉癌组织中的表达及其与喉癌部分临床生物学特性的相关性。方法:采用RTPCR方法检测GRO1在30例喉鳞状细胞癌组织(喉癌组)和10例正常喉黏膜组织(对照组)中的表达;免疫组织化学染色方法检测60例喉癌组织中GRO1和血管内皮生长因子(VEGF)的表达。结果:RTPCR结果显示,GRO1在喉癌组中的表达与对照组相比差异有统计学意义(P<0.01)。免疫组织化学染色结果显示,GRO1在有淋巴结转移的喉癌中的表达相对无淋巴结转移者差异有统计学意义(P<0.05);GRO1表达与喉癌分化程度无关(P>0.05);GRO1在不同年龄、性别、病变部位和不同T分期中的表达均无统计学意义(均P>0.05)。GRO1和VEGF的表达存在正相关关系(P<0.05)。结论:GRO1能够通过多种作用影响喉癌的发生、发展和转移,其中,在微血管形成方面与VEGF的高表达紧密关联。
Objective: To investigate the expression of growth-associated oncogene 1 (GRO1) in laryngeal carcinoma and its correlation with clinical characteristics of laryngeal cancer. METHODS: RT-PCR was used to detect the expression of GRO1 in 30 cases of laryngeal squamous cell carcinoma (laryngeal carcinoma group) and 10 cases of normal laryngeal mucosa (control group). Immunohistochemical staining was used to detect GRO1 in 60 cases of laryngeal carcinoma. Vascular endothelial growth factor (VEGF) expression. Results: RTPCR results showed that the expression of GRO1 in the laryngeal cancer group was significantly different from the control group (P<0.01). The results of immunohistochemical staining showed that the expression of GRO1 in lymph node metastasis of laryngeal carcinoma was significantly different from that of lymph node metastasis (P<0.05); the expression of GRO1 was not related to the degree of differentiation of laryngeal carcinoma (P>0.05); GRO1 was There was no significant difference in expression among different ages, genders, lesion sites, and different T stages (all P>0.05). There was a positive correlation between the expression of GRO1 and VEGF (P<0.05). Conclusion: GRO1 can affect the occurrence, development and metastasis of laryngeal cancer through multiple actions, among which, it is closely related to the high expression of VEGF in microvascular formation.