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目的探讨成骨生长肽羧基端片段[OGP(10-14)](G36G)及其类似物G48A对去卵巢(OVX)骨质疏松大鼠骨密度和骨生物力学的影响。方法 56只4月龄SD雌性大鼠分为7组,Ⅰ组为假手术组(Sham)术后给予磷酸盐缓冲液,Ⅱ～Ⅶ组行OVX手术,术后分别给予磷酸盐缓冲液(即OVX组)、阿仑膦酸钠(100μg/kg)、G36G(1或10 nmol/kg)和G48A(1或10 nmol/kg)。术后9周处死大鼠,采用放射免疫分析法检测骨钙素(BGP),测定股骨干重和灰重。腰椎和股骨行骨密度(BMD)检测。股骨行三点弯曲实验,腰椎行压缩实验,近端胫骨干骺行骨计量学测定。结果与OVX组相比,G48A高剂量组BGP显著升高,G36G和G48A各剂量组股骨灰重/干重比值和L1-4腰椎BMD均显著升高,G36G高剂量组和G48A各剂量组弯曲应力、骨小梁体积/全部骨体积、骨小梁体积/海绵骨体积和平均骨小梁板厚度显著增加,且平均骨小梁板间隙以及骨小梁面积和体积之比显著下降。结论 G36G及其类似物G48A可以延缓OVX大鼠的骨丢失,增加骨密度,提高骨生物力学性能。
Objective To investigate the effects of OGP (10-14)] (G36G) and its analogue G48A on bone mineral density and bone biomechanics in ovariectomized (OVX) rats. Methods Fifty-four SD rats of 4 months old were divided into 7 groups. Sham was given phosphate buffered saline (Group Ⅰ), OVX group Ⅱ-Ⅶ were given phosphate buffered saline OVX group), alendronate (100 μg / kg), G36G (1 or 10 nmol / kg) and G48A (1 or 10 nmol / kg). At 9 weeks after operation, rats were killed and BGP was measured by radioimmunoassay. The dry weight and gray weight of the femur were measured. Lumbar spine and femur bone mineral density (BMD) detection. Three-point bending test of femur, compression test of lumbar spine, proximal tibial epiphyseal bone measurement. Results Compared with OVX group, BGP in G48A high dose group was significantly increased, while the ratio of gray weight to dry weight and L1-4 lumbar spine BMD in G36G and G48A groups were significantly increased. The G36G high dose group and G48A dose group flexed Stress, trabecular volume / total bone volume, trabecular volume / sponge bone volume and mean trabecular thickness increased significantly with a significant decrease in mean trabecular plate clearance and trabecular area and volume. Conclusion G36G and its analogue G48A can delay the bone loss, increase the bone density and improve the biomechanical properties of OVX rats.