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目的观察Ｒｂ１对局灶性脑缺血区ＳＯＤ、ＮＯ含量的影响。方法ｗｉｓｔａｒ大鼠３０只体重３００－３５０ｇ，雌雄兼用，随机分为３组（ｎ＝１０）：①正常对照组（ＳＯ）；②大脑中动脉梗塞对照组（ＭＣＡＯ）；③药物组：２０μｇ／ｋｇ－１Ｒｂ１连续腹腔注射１０天。①②组生理盐水１０ｍｌ／ｋｇ－１连续腹腔注射１０天。于第１０天灌胃后３０ｍｉｎ①组动物处死取脑制成匀浆，②③组行ＭＣＡＯ后３０ｍｉｎ，处死取脑制成脑匀浆。根据ＮＯ与氧生成硝酸盐、亚硝酸盐，硝酸盐与磺酸及奈乙烯二胺酸盐反应为粉红偶氮化合物及酶联免疫吸附实验原理，采用比色法分别测定脑匀浆ＮＯ、ＳＯＤ含量。结果ＳＯ组脑匀浆ＮＯ含量为７．４１μｍｏｌ·ｍｇ－１（蛋白），ＳＯＤ为００２１μｍｇ·ｍｇ－１（蛋白）；ＭＣＡＯ组脑匀浆ＮＯ含量为１４７３μｍｏｌ·ｍｇ－１（蛋白），ＳＯＤ含量为００１９３ｍｇ·ｍｇ－１（蛋白）；Ｒｂ１组ＮＯ为７３６μｍｏｌ·ｍｇ－１（蛋白），ＳＯＤ含量为００２１９ｍｇ·ｍｇ－１（蛋白），各组间比较有显著差异（Ｐ＜０．０１）。结论①Ｒｂ１能降低ＭＣＡＯ区ＮＯ含量，降低ＮＯ含量的原理可能与ＳＯＤ有关。②Ｒｂ１能增加缺血脑组织ＳＯＤ含量。③脑中?
Objective To observe the effect of Rb1 on the content of SOD and NO in focal cerebral ischemia area. Methods Wistar rats weighing 300-350g were randomly divided into 3 groups (n = 10): ① normal control group (SO); ② middle cerebral artery infarction control group (MCAO); ③ drug group: 20μg / kg-1Rb1 continuous intraperitoneal injection of 10 days. ① ② group of normal saline 10ml / kg-1 continuous intraperitoneal injection for 10 days. On the 10th day after gavage 30min ① animals were sacrificed and homogenized brain, ② ③ group 30min after MCAO, sacrifice brain prepared brain homogenate. Nitric acid, nitrite, nitrite and sulfonic acid and neurethylenediamine were reacted with NO and oxygen to produce azo compounds and enzyme-linked immunosorbent assay. The contents of NO, SOD content. Results The content of NO in brain tissue of SO group was 7.41μmol · mg-1 (protein) and SOD was 0021μmg · mg-1 (protein). The content of NO in brain tissue of MCAO group was 1473μmol · mg- ), The content of SOD was 0193 mg · mg-1 (protein); the NO in Rb1 group was 736 μmol · mg-1 (protein), the SOD content was 0219 mg · mg-1 Significant difference (P <0.01). Conclusion (1) Rb1 can reduce the content of NO in MCAO area and reduce the content of NO. ② Rb1 can increase the content of SOD in ischemic brain tissue. ③ brain?