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目的：比较研究吗啡、哌替啶、芬太尼对离体人红细胞膜ＡＴＰａｓｅｓ活性的影响。方法：将不同浓度的药物分别与人红细胞孵育，以孔雀绿－磷钼酸复合比色法测定ＡＴＰａｓｅｓ活性。结果：随着药物浓度的增加，吗啡对Ｎａ＋，Ｋ＋－ＡＴＰａｓｅ活性有增强作用（Ｐ＜０．０５），芬太尼则呈明显地抑制作用（Ｐ＜０．０５），哌替啶仅具有轻微抑制作用；吗啡能明显抑制Ｍｇ２＋－ＡＴＰａｓｅ和Ｃａ２＋，Ｍｇ２＋－ＡＴＰａｓｅ活性（Ｐ＜０．０１或Ｐ＜０．０５），但哌替啶和芬太尼对Ｍｇ２＋－ＡＴＰａｓｅ活性均无影响；这两种药物仅轻微地抑制Ｃａ２＋，Ｍｇ２＋－ＡＴＰａｓｅ活性。结论：应用大剂量吗啡或芬太尼时，需注意他们对人红细胞膜ＡＴＰａｓｅｓ活性的影响。
OBJECTIVE: To compare the effects of morphine, meperidine and fentanyl on ATPase activity of isolated human erythrocyte membrane. Methods: The different concentrations of drugs were incubated with human erythrocytes respectively, and the activity of ATPases was determined by the malachite green - phosphomolybdic acid complex colorimetry. RESULTS: Morphine enhanced the activity of Na + and K + -ATPase (P <0.05) and decreased the activity of fentanyl (P <0.05) with the increase of drug concentration. Pethidine had only Morphine significantly inhibited the activity of Mg2 + -ATPase and Ca2 +, Mg2 + -ATPase (P <0.01 or P <0.05), but both pethidine and fentanyl had no effect on the activity of Mg2 + -ATPase; Both drugs only slightly inhibited Ca2 +, Mg2 + -ATPase activity. Conclusions: When using high doses of morphine or fentanyl, attention should be paid to their effect on ATPase activity of human erythrocyte membrane.