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目的通过测定兔血小板聚集和血栓素Ｂ２（ＴＸＢ２）、６－酮－前列腺素Ｆ_(Ⅰα)（６－ｋｅｔｏ－ＰＧＦ_(Ⅰα)）的含量，比较观察阿魏酸（Ｆ）、吲哚美辛（Ｉ）、阿魏酸－吲哚美辛结合物（ＦＩ）和阿魏酸－吲哚美辛－淀粉高分子载体衍生物（ＦＩＳ）在抗血栓方面的药理作用特点。方法用比浊法和放免法测定Ｆ、Ｉ、ＦＩ和ＦＩＳ在体外对兔血小板聚集和对ＴＸＢ２，６－ｋｅｔｏ－ＰＧＦ_(１α)生成的影响。结果Ｆ、Ｉ、ＦＩ和ＦＩＳ显著地抑制ＡＤＰ和ＡＡ诱导的兔血小板聚集和ＴＸＢ_２，６－ｋｅｔｏ－ＰＧＦ_(Ⅰα)的产生。在 １２．５－２４０μｍｏｌ／Ｌ的剂量范围内，对血小板聚集的抑制率， Ｆ为 １１．３％－３１．８％（ＡＤＰ诱导）和 ３.３％－１８．５％（ＡＡ诱导）、Ｉ为３％－３４．３％（ＡＤＰ诱导）和２％－２０．２％（ＡＡ诱导）、ＦＩ为５．０％－５９．３％（ＡＤＰ诱导）和０．３％－６３．１％（ＡＡ诱导）、ＦＩＳ为３.１％－３９．１％（ＡＤＰ诱导）和１．５％－３９.６％（ＡＡ诱导）。剂量为 ２４０.０μｍｏｌ／Ｌ时对ＴＸＢ_２和６－ｋｅｔｏ－ＰＧＦ_(Ⅰα)产生的抑制率， Ｆ为 １４．８％和 １.２％、Ⅰ为 ２４.９％和 ２６．２％、 ＦＩ为 ４０.６％和２９．３％?
OBJECTIVE: To investigate the effect of ferulic acid (F), indomethacin (TXB2) and 6-keto-PGF_ (Iα) (I), pharmacological effects of ferulic acid-indomethacin conjugates (FI) and ferulic acid-indomethacin-starch polymer carrier derivatives (FIS) Methods The turbidimetric and radioimmunoassay methods were used to determine the effect of F, I, FI and FIS on platelet aggregation and TXB2, 6-keto-PGF_ (1α) production in vitro. Results F, I, FI and FIS significantly inhibited ADP and AA-induced rabbit platelet aggregation and the production of TXB_2, 6-keto-PGF_ (Ⅰα). The inhibition of platelet aggregation was 11.3% -31.8% (ADP induction) and 3.3% -18.5% (AA induction) in the dose range of 12.5-240 μmol / L, I was 3% -34.3% (ADP induction) and 2% -20.2% (AA induction), FI was 5.0% -59.3% (ADP induction) and 0.3% -63.1 % (AA induction), FIS 3.1% -39.1% (ADP induction) and 1.5% -39.6% (AA induction). The inhibitory rates of TXB_2 and 6-keto-PGF_ (Ⅰα) at doses of 240.0μmol / L were 14.8% and 1.2% for F, 24.9% and 26.2% for I, and FI was 40.6% and 29.3%?