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目的 :观察大白鼠回肠肌间神经丛一氧化氮合酶阳性神经元缺血后的变化。方法 :阻断大白鼠肠系膜上动脉 ,用还原型尼克酰胺腺嘌呤二核苷酸脱氢酶反应 (NADPH—d)法 ,观察大鼠回肠肌间神经丛一氧化氮合酶阳性神经元的变化及神经损伤。结果 :肠缺血 3 0min ,大鼠回肠肌间神经丛内一氧化氮合酶阳性神经元数目无明显变化 ,但染色深度加强 ;缺血 1小时及 2小时 ,一氧化氮合酶阳性神经元胞体明显增多而且染色加深。结论 :一氧化氮 (No)在肠缺血所致的神经损伤中起着重要作用。
Objective: To observe the changes of nitric oxide synthase positive neurons in the myenteric plexus of rats after ischemia. Methods: The superior mesenteric artery was occluded in rats and the changes of nitric oxide synthase (NOS) positive neurons in the myenteric plexus of rat were observed by NADPH-d And nerve damage. Results: The numbers of nitric oxide synthase positive neurons in the myenteric plexus of rats after 30min of intestinal ischemia had no significant changes, but the depth of staining was enhanced. At 1 hour and 2 hours after ischemia, nitric oxide synthase positive neurons Significantly increased cell body and staining deepened. Conclusion: Nitric oxide (NO) plays an important role in nerve injury induced by intestinal ischemia.