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目的 :探讨围手术期胰腺癌患者细胞免疫功能的表达。方法 :用直接免疫荧光技术和流式细胞仪检测 36例胰腺癌患者介入治疗前后和手术前后外周血T淋巴细胞亚群CD3+ 、CD4+ 、CD8+ 和NK细胞的百分率。结果 :胰腺癌患者外周血CD3+ 、CD4+ 百分率和CD4+ /CD8+ 比值 ,与对照组相比明显低下 ,在介入治疗后 1周下降更显著 ,但在手术前 (介入后 3～ 4周 )有所恢复 ,在手术后出现再次下降 ;NK细胞比率变化无显著差别。结论 :胰腺癌患者的细胞免疫功能表达低下 ,介入化疗和手术创伤可加重抑制细胞免疫功能的表达 ,临床上应注意围手术期细胞免疫功能的保护
Objective: To investigate the expression of cellular immune function in patients with perioperative pancreatic cancer. Methods: The percentage of T lymphocyte subsets CD3 +, CD4 +, CD8 + and NK cells in peripheral blood of 36 patients with pancreatic cancer before and after interventional therapy and before and after surgery were detected by direct immunofluorescence and flow cytometry. Results: The ratio of CD3 +, CD4 + and CD4 + / CD8 + in peripheral blood of patients with pancreatic cancer was significantly lower than that of the control group. The decrease was more significant at 1 week after interventional treatment, but was restored before surgery (3-4 weeks after intervention) , After surgery again declined; NK cell ratio was no significant difference. Conclusions: The expression of cellular immune function in patients with pancreatic cancer is low, the expression of cellular immune function may be inhibited by interventional chemotherapy and surgical trauma, and the protection of cellular immune function in perioperative period should be paid attention to