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目的探讨α-硫辛酸(LA)对糖基化终产物(AGEs)作用下的人脐静脉血管内皮细胞株(HUVEC)表面程序性死亡配体1(PD-L1)分子表达及培养上清液中可溶性PD-L1(sPD-L1)浓度的影响。方法实验分四组:正常对照组(C组:RPMI1640培养);糖基化牛血清白蛋白(AGE-BSA)组[培养液分别为AGE-BSA50mg/ml(AG50组)、200mg/ml(AG200组)和400mg/ml(AG400组)];牛血清白蛋白(BSA)对照组[培养液分别为BSA50mg/ml(BG50组)、200mg/ml(BG200组)和400mg/ml(BG400组)];药物干预组[培养液分别为RPMI1640+200μmol/LLA(C+LA200组)、200mg/mlAGE-BSA+200μmol/LLA(AG200+LA200组)]。孵育72h后,检测各组HUVEC细胞表面PD-L1的表达及上清液中sPD-L1浓度的变化。结果不同浓度AGE-BSA作用细胞72h,PD-L1的表达及sPD-L1浓度较对照组显著升高(P<0.01),其效应呈现一定的浓度依赖性。在LA干预下,AG200+LA200组PD-L1表达及sPD-L1浓度显著低于AG200组(P<0.05)。结论 AGEs能有效上调内皮细胞表面PD-L1表达,并增加培养上清液中sPD-L1浓度;而LA则可抑制AGEs的诱导作用。
Objective To investigate the molecular expression of PD-L1 on human umbilical vein endothelial cell line (HUVEC) induced by α-lipoic acid (LA) and advanced glycation end products (AGEs) Effect of Soluble PD-L1 (sPD-L1) Concentration. Methods The experiment was divided into four groups: normal control group (C group: RPMI1640 culture); AGE-BSA group (AGE-BSA50mg / ml AG50 group, AG200 group (BG400 group), 200mg / ml (BG200 group) and 400mg / ml (BG400 group)], respectively; ; Drug intervention group [culture medium was RPMI1640 + 200μmol / LLA (C + LA200 group), 200mg / ml AGE + BSA + 200μmol / LLA group]. After 72 hours of incubation, the PD-L1 expression on the surface of HUVECs and the concentration of sPD-L1 in the supernatant were detected. Results After treated with AGE-BSA for 72 h, the PD-L1 expression and sPD-L1 concentration were significantly increased (P <0.01), and the effect was dose-dependent. Under LA intervention, PD-L1 expression and sPD-L1 concentration in AG200 + LA200 group were significantly lower than those in AG200 group (P <0.05). Conclusion AGEs can effectively up-regulate the expression of PD-L1 on endothelial cells and increase the concentration of sPD-L1 in culture supernatant. LA can inhibit the induction of AGEs.