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目的 MTT法检测细胞因子信号负调控因子3(suppressor of cytokine signaling 3,SOCS3)稳转乳腺癌细胞株对他莫昔芬(tamoxifen,TAM)的耐药性。方法用慢病毒LV-SOCS3(5775-1)感染人乳腺癌MDA-MB-231细胞,经氨苄西林筛选及扩增后,建立SOCS3稳转乳腺癌细胞株。常规培养正常及SOCS3稳转人乳腺癌MDA-MB-231细胞,并分别加入不同浓度的TAM(0、10、20、30、40μmol/L),采用MTT法检测各组细胞的相对增殖率。结果相同TAM浓度下,SOCS3稳转人乳腺癌MDA-MB-231细胞的相对增殖率明显低于人乳腺癌MDA-MB-231细胞(P<0.05)。结论 SOCS3稳转细胞株对TAM耐药性显著降低,为内分泌治疗耐药的乳腺癌患者提供了一种新的治疗方法。
Objective To detect the resistance of tumor suppressor of cytokine signaling 3 (SOCS3) to tamoxifen (TAM) in human breast cancer cells by MTT assay. Methods Human breast cancer MDA-MB-231 cells were infected with lentivirus LV-SOCS3 (5775-1). After ampicillin was screened and expanded, SOCS3 stable breast cancer cell lines were established. Routine normal culture and SOCS3 metastasis of human breast cancer MDA-MB-231 cells were added different concentrations of TAM (0,10,20,30,40 μmol / L), the relative proliferation rate of each group of cells was detected by MTT assay. Results The relative proliferation rate of SOCS3 stable human breast cancer MDA-MB-231 cells was significantly lower than that of human breast cancer MDA-MB-231 cells at the same concentration of TAM (P <0.05). CONCLUSIONS: SOCS3 stable cell lines have a significantly lower resistance to TAM and provide a new therapeutic approach for endocrine-resistant breast cancer patients.