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目的探讨以胸腺肽α1(Tα1)为基础,联合槐耳颗粒、西罗莫司的三联抗肿瘤疗法对大鼠肝癌模型血清甲胎蛋白(AFP)水平的影响。方法 90只SD大鼠以随机数字法分为Tα1组、槐耳颗粒组、西罗莫司组、三联组、诱癌组和正常对照组,每组15只。除正常对照组外,其余各组均采用化学诱癌法建立SD大鼠肝癌动物模型,自DEN开始处理后即开始用药,三联组采用0.8 mg/kg Tα1皮下注射,开始每日1次,2周后改为每周2次;0.35 g/kg槐耳颗粒灌胃,3次/日;1 mg/kg西罗莫司灌胃,1次/日。其余各单独用药组的用药剂量同三联组,诱癌组和对照组不予以药物治疗,用药持续到第20周。观察各组大鼠在不同时期的行为表现,并检测各组大鼠6、16、18、20周的血清AFP的水平。结果经诱癌处理的各组大鼠在第16周表现出典型的肝癌症状,并从第10周开始相继死去6只,解剖出各只大鼠肝脏,肉眼观察可见肝脏表面不光滑且有结节。肝脏病理检查证实肝癌模型诱导成功。根据第20周存活大鼠的肝癌发生率,表明三联疗法的大鼠肝癌发生率显著低于其他处理组,存活率显著高于其他处理组。三联疗法显著降低大鼠体内血清AFP水平。结论三联疗法能显著延长患癌大鼠的生存时间、降低发癌率和血清AFP水平。
Objective To investigate the effect of triple anti-tumor therapy combined with Huaier granule and sirolimus on the level of serum alpha-fetoprotein (AFP) in rat hepatocarcinoma model based on thymosin α1 (Tα1). Methods 90 SD rats were randomly divided into Tα1 group, Huaier granule group, sirolimus group, triple group, cancer-inducing group and normal control group, with 15 rats in each group. Except for the normal control group, the other groups were established by using the method of chemical induced cancer to establish an animal model of hepatocellular carcinoma in SD rats. The treatment started from the beginning of DEN treatment, and the triple therapy group received 0.8 mg / kg Tα1 subcutaneously once daily for 2 Week to 2 times a week; 0.35 g / kg Huaier particles gavage, 3 times / day; 1 mg / kg sirolimus orally, 1 / day. The rest of the medication alone group with the triple dose group, the cancer-free group and the control group were not drug treatment, medication continued until the 20th week. The behavior of rats in each group was observed at different times, and the levels of serum AFP in 6, 16, 18 and 20 weeks of rats in each group were detected. Results All the rats induced by cancer treatment showed typical symptoms of liver cancer at the 16th week. Six rats died from the 10th week after operation, and the liver of each rat was dissected. The naked eye showed that the surface of the liver was not smooth and had a knot Festival. Liver pathology confirmed the successful induction of liver cancer model. According to the incidence of liver cancer in rats surviving at the 20th week, the incidence of liver cancer in the triple therapy group was significantly lower than that of other treatment groups, and the survival rate was significantly higher than that of other treatment groups. Triple therapy significantly reduced serum AFP levels in rats. Conclusion Triple therapy can significantly prolong the survival time of cancer-bearing rats, reduce the incidence of cancer and serum AFP levels.