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我们通过培养来源于慢性胃炎患者胃液的染色曲霉,分离制备了包括杂色曲霉素(ST)、averufin(AV)和新产物5′-hydroxy-averantin(5′-HA)等代谢产物。并检测它们及其有关衍生物的致突变性。Ames试验表明:在0.1～100μg/皿剂量时,ST、O—甲基—ST、O—乙酰基—ST,不论有无S_θ存在,都显示阳性结果,而AV和5′-HA只是在S_θ存在时出现阳性;加S_θ时5′-HA和AV对TA_(θ8)和TA_(100)菌株的致突变性强度分别是AFB_1的0.02％和0.03％、0.04％,而ST则是13.75％和38.35％。在S_θ存在时ST、5′-HA、AV对CHL细胞染色体畸变阳性剂量分别是0.05、10.0、50.0μg/ml,不加S_θ,只有ST在5μg/ml时就出现阳性。染色体畸变类型,ST和AV以cte为主。而5′-HA以ctb为主。据此认为新产物5′-HA和AV的致突变性,远远弱于ST和AFB_1。
We isolated and prepared metabolites that include Aspergillus fumigatus (ST), averufin (AV) and a novel product 5’-hydroxy-averantin (5’-HA) by culturing A. oryzae derived from the gastric juice of patients with chronic gastritis. And detecting the mutagenicity of them and their related derivatives. Ames test showed that ST, O-methyl-ST, O-acetyl-ST, with or without S_θ, showed positive results at the dose of 0.1-100 μg / dish, while AV and 5’-HA only showed positive results at S_θ The positive mutagenicity of 5’-HA and AV to TA_ (θ8) and TA_ (100) strains at addition of S_θ was 0.02% and 0.03%, 0.04% and that of ST was 13.75% and 38.35%. The positive doses of ST, 5’-HA and AV to chromosomal aberrations of CHL cells in the presence of S_θ were 0.05, 10.0 and 50.0 μg / ml, respectively, without S_θ, but only ST was positive at 5 μg / ml. Chromosomal aberrations type, ST and AV to cte-based. While 5’-HA is mainly ctb. Accordingly, the mutagenicity of the novel products 5’-HA and AV is considered to be much weaker than that of ST and AFB_1.