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目的和方法 :通过对大鼠侧脑室微量注射NOS抑制剂L NAME及NO的前体L 精氨酸 (L Arg)观察两种物质对大鼠睡眠 觉醒的影响。结果 :注射 1mgL NAME(5 μl)后 4h觉醒 (W )明显增加 ,尤以注射后第 1～ 2h显著 ;4h慢波睡眠 (SWS)明显减少 ,该效应同样以注射后第 1～ 2h显著 ;异相睡眠 (PS)无明显变化。小剂量L NAME(0 .2mg ,5 μl)对大鼠的W、SWS、PS无明显影响 ;同样方法注射NO前体L Arg 30 0 μg/ 5 μl3～ 4h后W明显减少而SWS显著增加 ;大鼠侧脑室注射L Arg(30 0 μg/ 5 μl) +L NAME(1mg/ 5 μl)后 ,W、SWS及PS无明显变化。 结论 :L NAME通过抑制NOS使大鼠W增加 ,SWS减少 ,这一作用可被NO前体L Arg所拮抗 ,说明NO参与了机体睡眠 -觉醒的调节
PURPOSE AND METHODS: The effects of two substances on sleep arousal in rats were observed by microinjection of NOS inhibitor L NAME into the lateral ventricle and L-arginine (NO) precursor. Results: Awakening (W) increased significantly at 4h after injection of 1mgL NAME (5μl), especially at 1 ~ 2h after injection. The SWS decreased significantly at 4h after injection, which was also marked at 1 ~ 2h after injection. Heterogeneous sleep (PS) no significant change. Low dose of L NAME (0.2 mg, 5 μl) had no significant effect on W, SWS and PS in rats. Similarly, NO decreased significantly after W / L 30 μg / After intracerebroventricular injection of L Arg (30 0 μg / 5 μl) + L NAME (1 mg / 5 μl), there was no significant change in W, SWS and PS. CONCLUSION: L NAME can increase W and decrease SWS in rats by inhibiting NOS, which can be antagonized by NO precursor L Arg, indicating that NO participates in the regulation of sleep-wakefulness