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目的 评价大鼠脑缺血后的学习、记忆功能及其与海马组织学变化的关系。方法 用Morris全自动新式水迷宫试验方法对反复脑缺血再灌注大鼠进行学习获得试验、记忆保持试验 ,光镜观察海马组织学变化 ,并计算海马神经元密度。结果 与对照组比较 ,反复脑缺血大鼠学习记忆成绩显著下降 ,潜伏期显著延长 ,游水迷宫形呈周边型 ;缺血后海马CA1、CA4 和PM区神经元大量丧失。结论 大鼠反复脑缺血出现严重的学习、记忆障碍。认知功能改变与海马神经元缺血性损害有关。用Morris全自动水迷宫评价缺血所致学习、记忆障碍简便、准确、真实。
Objective To evaluate the learning and memory function after cerebral ischemia in rats and its relationship with histological changes in hippocampus. Methods Morris automatic new water maze test was used to study the rats with repeated cerebral ischemia-reperfusion, and the memory retention test was carried out. The histopathological changes of hippocampus were observed with light microscope and the density of hippocampal neurons was calculated. Results Compared with the control group, the scores of learning and memory in the rats with recurrent cerebral ischemia decreased significantly, the latent period significantly prolonged, and the swimming labyrinth showed the peripheral type. After ischemia, neurons in CA1, CA4 and PM of hippocampus were greatly lost. Conclusion There is serious learning and memory impairment in recurrent cerebral ischemia in rats. Changes in cognitive function and hippocampal neuronal ischemic damage related. Using Morris automatic water maze evaluation of ischemia-induced learning, memory disorders is simple, accurate and true.