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目的:综述飞行质谱技术在早期消化道肿瘤研究中的应用。方法:以“癌前病变、蛋白质组学、飞行时间质谱、肿瘤、肿瘤标志”为关键词,运用pubMed central及CNKI期刊全文数据库检索2000-2011有关文献,初步筛选150余篇相关文献,纳入标准:1)消化道肿瘤相关流行病学调查现状;2)飞行时间质谱在食管癌、胃癌及大肠癌早期中的研究现状。根据纳入标准,纳入分析26篇相关文献。结果:大量研究结果证明飞行时间质谱技术的灵敏度及特异性均>85%,能够发现早期肿瘤组织或体液当中传统技术所不能发现的差异蛋白改变,为消化道肿瘤的发生发展、早期诊断及临床筛查提供新的途径。结论:质谱技术将成为早期消化道肿瘤蛋白变化研究的主要技术之一。
Objective: To summarize the application of flight mass spectrometry in the study of early gastrointestinal tumors. Methods: Using the keywords “precancerous lesion, proteomics, time of flight mass spectrometry, tumor and tumor marker” as the key words, pubMed central and CNKI periodical full-text databases were used to search 2000-2011 related literature and preliminary screening more than 150 articles, Inclusion criteria: 1) prevalence of gastrointestinal tumor epidemiological survey; 2) time of flight mass spectrometry in esophageal cancer, gastric cancer and early stage of colorectal cancer research. According to inclusion criteria, included in the analysis of 26 related articles. Results: A large number of studies have demonstrated that the time-of-flight mass spectrometry has a sensitivity and specificity of> 85% and can detect differences in proteins that can not be detected by traditional techniques in early tumor tissues or body fluids. It is useful for the diagnosis, development and early diagnosis of gastrointestinal tumors Screening provides new avenues. Conclusion: Mass spectrometry technology will become one of the main technologies in the study of early gastrointestinal cancer protein changes.