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目的 :观察小柴胡汤及其分解剂Ⅰ号和分解剂Ⅱ号对心肌细胞的保护和对机体的免疫调节作用。方法 :以柯萨奇病毒B3亲心肌株 (CVB3m)腹腔注射诱导Balb/c乳鼠心肌炎模型 ,分别予以小柴胡汤、分解剂Ⅰ号及分解剂Ⅱ号 ,经灌胃后 ,于不同时间进行各组的NK细胞活性、T细胞亚群及心肌病理组织学检查。结果 :(1)小柴胡汤和分解剂Ⅱ号在病毒性心肌炎急性期均能明显提高NK细胞活性 ,有调节T细胞亚群功能 ,与病毒对照组比较有显著性差异 (P <0 0 5)。而分解剂Ⅰ号此作用不明显。 (2 ) 3种治疗药物对心肌免疫损伤均有明显保护作用 ,小柴胡汤作用明显优于分解Ⅰ号和分解剂Ⅱ号 (P <0 0 5)。而分解剂Ⅰ号和分解剂Ⅱ号比较未见显著性差异 (P >0 0 5)。结论 :小柴胡汤和分解剂Ⅱ号对病毒性心肌炎NK细胞活性和T细胞亚群具有双向调节作用 ,3种治疗药物对心肌的免疫损伤均有明显保护作用。
Objective : To observe the protective effects of Xiaochaihu Decoction and its decomposing agent I and decomposing agent II on cardiomyocytes and immune regulation of the body. METHODS: The Balb/c neonatal rat myocarditis model was induced by intraperitoneal injection of Coxsackievirus B3 parental cardiomyocytes (CVB3m). The mice were treated with Xiaochaihu Decoction, Decomposition Agent I and Decomposition Agent II respectively. After intragastric administration, they were administered at different times. The NK cell activity, T cell subsets, and histopathological examination of the myocardium of each group. Results: (1) Xiaochaihu Decoction and Decomposition Agent II can significantly increase the activity of NK cells in the acute phase of viral myocarditis and regulate the function of T-cell subpopulations. There is a significant difference compared with the virus control group (P <0 0 5 ). The decomposer I has no obvious effect. (2) The three therapeutic drugs all had significant protection against myocardial immunological injury. Xiaochaihu Decoction was significantly better than Decomposition I and Decomposition II (P <0 05). There was no significant difference between Decomposition Agent I and Decomposition II (P > 0 05). Conclusion : Xiaochaihu Decoction and Decomposition Agent II have two-way adjustment effect on NK cell activity and T cell subsets of viral myocarditis. The three therapeutic drugs have obvious protective effects on the immune damage of myocardium.