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目的探讨西洋参多糖对钴-60γ射线照射致小鼠免疫抑制模型的免疫调节作用,并初步探讨其作用机制。方法将180只6～8周龄、18～22 g的雌性BALB/c小鼠随机分为5组:空白对照组、模型对照组和3个西洋参多糖剂量组(50mg/kg BW、100mg/kg BW和200mg/kg BW)。除空白对照组外,其余4组于d45,一次性接受全身照射(γ射线总剂量为4Gy)。3 d后,测定脾脏T淋巴细胞增殖能力、迟发型变态反应程度和碳粒廓清实验,测定血清丙二醛水平、超氧化物歧化酶活性和谷胱甘肽过氧化物酶活性。结果西洋参多糖在3个剂量组均能明显升高小鼠T淋巴细胞的增殖能力和迟发型变态反应程度,在100mg/kg BW和200mg/kg BW组能明显增高小鼠碳粒廓清指数,在3个剂量组均能明显降低小鼠血清中MDA水平,在50mg/kg BW组能明显增高小鼠血清SOD活性,小鼠血清GSH-Px活性差异无统计学意义。结论西洋参多糖能增强钴-60γ射线照射致免疫抑制小鼠免疫功能,可能通过减轻氧化应激对免疫系统的损害来实现。
Objective To investigate the immunomodulatory effect of polysaccharide from American ginseng on immunosuppression induced by cobalt-60γ-ray in mice and to explore its mechanism. Methods 180 female BALB / c mice aged 6 ~ 8 weeks and 18 ~ 22 g were randomly divided into 5 groups: control group, model control group and three polysaccharides groups (50mg / kg BW, 100mg / kg BW and 200 mg / kg BW). In addition to the blank control group, the remaining four groups in d45, a one-time whole body irradiation (γ-ray total dose of 4Gy). After 3 days, the proliferation ability of T lymphocytes, the degree of delayed-type hypersensitivity reaction and carbon clearance test were measured. The levels of malondialdehyde, superoxide dismutase and glutathione peroxidase were measured. Results Panax ginseng polysaccharide could significantly increase the T lymphocyte proliferation rate and the degree of delayed-type hypersensitivity in three dose groups, and significantly increase the carbon clearance index of mice in 100 mg / kg BW and 200 mg / kg BW groups All the three dose groups could significantly reduce the level of MDA in the serum of mice, and the activity of SOD in serum of mice was obviously increased in 50mg / kg BW group. There was no significant difference in the activity of GSH-Px in mice. Conclusion The polysaccharides from American ginseng can enhance the immune function of immunosuppressed mice induced by cobalt-60γ-ray irradiation, which may be achieved by reducing the damage of oxidative stress to the immune system.