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作者研究了用持续感染1型人类免疫缺陷病毒(HIV-1)的HeLaT4~+细胞在间接免疫荧光抗体测定(IFA)中的适应性,以探索用其作为HIV 血清学中IFA 证实试验病毒抗原的替代来源。HIV-1持续感染培养物的方法是,将未感染HeLaT4~+细胞悬液与/mlHIV-1感染H9细胞液(传代后72小时)混合并接种于25cm~2烧瓶。72小时后,除去培养液并用生长培养液洗涤细胞层除去H9细胞碎片。这时,细胞表现有合胞体形成的广泛细胞病变作用。向细胞添加新鲜生长培养液并再孵育。每4天换一次生长培养液共14天,使细胞建立起融
The authors studied the suitability of HeLaT4 ~ + cells in continuous immunization with human immunodeficiency virus type 1 (HIV-1) in indirect immunofluorescent antibody assay (IFA) to explore the potential role of IFA in testing IFA in HIV serology Alternative source. Cultures were continuously infected with HIV-1 by mixing uninfected HeLaT4 ~ + cell suspensions with / ml HIV-1 infected H9 cell pellets (72 hours after passage) and inoculated into 25 cm 2 flasks. After 72 hours, the culture broth was removed and the cell layer was washed with growth medium to remove H9 cell debris. At this time, the cells show extensive cytopathic effects of syncytia formation. Add fresh growth medium to the cells and incubate again. Every 4 days for a total of 14 days growth medium, so that the establishment of cell fusion