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托盘根乙醇提取物体外实验能显著抑制大鼠肝、脑、脾、肾组织中过氧化脂质(LPO)为生成。1.51g/kg、0.755g/kg、ig连续15天能抑制小鼠血浆、脑、肝中过氧化脂质的生成,并能降低其肝、心脂褐素(LPF)含量。提示托盘根醇提物具有抗组织过氧化作用。P<0.05*P<0.01结果(表2)托盘根醇提物高、低剂量组均能显著抑制小鼠血浆过氧化脂质的生成。2.3对小鼠肝、脑匀浆过氧化脂质生成的影响:分组及给药同上。第16天断头处死,取肝、脑按文献[4]处理后测吸光度。以四乙氧基丙烷为标准计算每克组织过氧化脂质的生成量。表3托盘根醇提物对小鼠肝、脑匀浆过氧化脂质生成的影响与NS组比较P<0.01结果(表3)托盘根醇提物高、低剂量体内给药均能显著抑制小鼠肝、脑匀浆过氧化脂质的生成。2.4对小鼠肝、心脂褐素含量的影响:分组及给药同上。第16天断头处死,取肝、心按文献[4]测LPF。以0.05mol/L硫酸新鲜配制的0.1mg/L硫酸奎宁的荧光强度为50单位,以每克湿组织含μg硫酸奎宁表示。表4托盘根醇提物对小鼠肝、心LPF含量的影响与NS组比较P<0.01结果(表4)托盘根醇提物对小鼠肝、心LPF的生成有显著抑制作?
Ethanol extraction from tray roots significantly inhibited the production of lipid peroxidation (LPO) in liver, brain, spleen, and kidney of rats. 1.51g/kg, 0.755g/kg, ig for 15 consecutive days could inhibit the production of lipid peroxides in plasma, brain and liver of mice, and reduce the liver and heart lipofuscin (LPF) levels. It suggests that the alcohol extract of the tray root has anti-tissue peroxidation. P<0.05*P<0.01 Results (Table 2) High and low doses of alcohol extracts from the roots of trays all significantly inhibited lipid peroxide production in mice. 2.3 Effect of Lipid Peroxidation on Mouse Liver and Brain Homogenization: Grouping and Administration Same as above. On the 16th day, the rats were killed by decapitation and the absorbances of the liver and brain were measured according to the literature [4]. The production of lipid peroxide per gram of tissue was calculated based on tetraethoxypropane. Table 3 Effect of alcohol extracts from trays on the lipid peroxidation of liver and brain homogenates in mice Compared with NS group P<0.01 Results (Table 3) High and low doses of alcohol extracts from tray root can be administered in vivo Significantly inhibited mouse liver and brain homogenate lipid peroxide production. 2.4 The effect of liver and heart lipofuscin content in mice: grouping and administration were the same as above. On the 16th day, the rats were decapitated and the liver and heart were taken as LPF according to the literature [4]. The fluorescence intensity of 0.1 mg/L quinine sulfate freshly prepared with 0.05 mol/L sulfuric acid was 50 units, and was expressed as μg of quinine sulfate per gram of wet tissue. Table 4 Effect of alcohol extracts from trays on liver and heart LPF content in mice Compared with NS group P<0.01 Results (Table 4) Ethanol extracts from tray root significantly inhibit the production of liver and heart LPF in mice.