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选育耐密紧凑株型是增加玉米单位面积产量的重要途径之一,而叶夹角和叶向值是衡量株型的重要参数。本研究选用叶夹角和叶向值存在差异的玉米自交系郑58、PH6WC、87-1和自330构建1个四交(郑58/豫87-1//PH6WC/自330)组合,以228个四交F1单株为作图群体,构建了1张含225个SSR位点,全长1 387.2cM的玉米分子标记遗传连锁图谱,标记间平均间距为6.19cM。基于四交群体应用区间作图法检测4个环境下的QTL,共检测到13个叶夹角相关QTL,分别位于第1、2、3、4、5、7和10染色体上,单个QTL可解释5.1%~20.0%的表型变异;检测到15个叶向值相关QTL,分别位于第1、2、4、5、7、8和9染色体上,单个QTL可解释5.4%~20.1%的表型变异。其中qLA-E2-2和qLA-E4-2落在同一标记区间umc1692-umc2297(bin 5.03),分别解释16.6%和13.2%的表型变异;qLO-E1-1、qLO-E3-2和qLA-E4-1落在同一标记区间umc1568-bnlg1953(bin1.02),分别解释10.1%、19.9%和12.3%的表型变异;qLO-E2-1和qLO-E3-1落在同一标记区间phi056-phi427913(bin 1.01),分别解释13.8%和10.0%的表型变异。这些多个环境共同检测到的QTL将为玉米耐密理想株型育种中叶夹角叶向值的分子标记辅助选择提供有益信息,加速耐密株型玉米品种的选育。
Breeding compact compact plant type is to increase yield per unit area of corn is one of the important ways, and leaf angle and leaf value is an important parameter to measure plant type. In this study, maize inbred lines Zheng 58, PH6WC, 87-1 with different leaf included angle and leaf orientation were selected and a combination of four crosses (Zheng 58 / Yu 87-1 // PH6WC / self 330) A total of 228 SSR F1 plants were used as a mapping population to construct a maize molecular marker linkage map with 225 SSR loci and a total length of 1 387.2 cM. The average spacing between markers was 6.19 cM. Four QTLs for leaf angle were detected in four environments based on cross-fertilization interval mapping, and QTLs were detected on chromosomes 1, 2, 3, 4, 5, 7 and 10 respectively. Explained the phenotypic variation from 5.1% to 20.0%. Fifteen QTLs for leaf value were detected on chromosomes 1, 2, 4, 5, 7, 8 and 9 respectively. A single QTL explained 5.4% -20.1% Phenotypic variation. Among them, qLA-E2-2 and qLA-E4-2 fell within the same marker interval umc1692-umc2297 (bin 5.03), respectively, accounting for 16.6% and 13.2% of phenotypic variation; qLO-E1-1, qLO-E3-2 and qLA -E4-1 fall in the same marker interval umc1568-bnlg1953 (bin1.02), respectively, explaining 10.1%, 19.9% and 12.3% of phenotypic variation; qLO-E2-1 and qLO-E3-1 fall in the same marker interval phi056 -phi427913 (bin 1.01) explaining 13.8% and 10.0% of the phenotypic variation, respectively. The QTLs detected by these multiple environments will provide useful information for the molecular marker-assisted selection of mid-angle leaf orientation in maize plants with close tolerance, and accelerate the selection and breeding of tight-tolerant maize varieties.