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斯卑尔脱小麦材料Altgold含有小麦抗叶锈病基因LrAlt。该基因被定位于小麦2A染色体短臂末端。本研究基于小麦与短柄草和水稻基因组良好的共线性关系,对小麦抗叶锈病基因LrAlt进行比较基因组学分析,发现该基因所在基因组区域对应于短柄草第5染色体和水稻第4染色体的直系同源基因组区域,据此开发出与LrAlt连锁的EST-STS标记BE498683、BE471132.1、BG605273和CD454629,并构建了LrAlt的遗传连锁图谱,这4个EST-STS标记与Xbarc212共分离,位于LrAlt近着丝粒侧,距离LrAlt 1.9cM。同时,通过筛选Graingenes 2.0公布的位于LrAlt附近的SSR标记,发现Xbarc124、Xgwm614与LrAlt紧密连锁,均与Xbarc212共分离。本研究通过比较基因组学的策略和筛选Graingenes 2.0公布的SSR标记,共得到与LrAlt紧密连锁的9个新的分子标记,为构建LrAlt的高密度精细遗传连锁图谱、分子标记辅助选择和基因聚合奠定了基础。
Spelled wheat material Altgold contains the wheat leaf rust resistance gene LrAlt. The gene is located on the short arm of chromosome 2A in wheat. In this study, based on the good collinearity between wheat and Brassica chinensis and rice genome, a comparative genomics analysis of wheat leaf rust resistance gene LrAlt was carried out and it was found that the genomic region of the gene corresponded to the fifth chromosome and the fourth chromosome of rice Orthologous genomic regions, EST-STS markers BE498683, BE471132.1, BG605273 and CD454629 linked to LrAlt were developed and a genetic linkage map of LrAlt was constructed. These four EST-STS markers were co-segregated with Xbarc212, LrAlt proximal centromere, distance LrAlt 1.9cM. At the same time, Xbarc124 and Xgwm614 were found to be closely linked to LrAlt by screening SSR markers located in the vicinity of LrAlt in Graingenes 2.0, all of which were co-segregated with Xbarc212. In this study, nine new molecular markers closely linked to LrAlt were obtained by comparing genomics strategies and screening SSR markers published by Graingenes 2.0, which laid the foundation for the construction of high-density fine genetic linkage map, molecular marker-assisted selection and gene polymerization in LrAlt The foundation.