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目的研究EB病毒Ig G、Ig A、Ig M和EB-DNA检测在儿童呼吸道感染性疾病中的诊断价值。方法采用酶联免疫吸附法(ELISA法)检测湖南省人民医院431例呼吸道感染患儿的血清EB病毒抗体,同时用荧光探针PCR法检测患儿咽拭子或血浆EB-DNA;另选该院同期189例无呼吸道感染住院患儿为对照组。分析呼吸道感染患儿不同年龄段EB-DNA和EBVCA三种抗体阳性率差异,并通过ROC曲线分析比较各项目在呼吸道感染性疾病诊断中的效能。结果EB-DNA和Ig G、Ig M两种抗体阳性率在不同年龄段呼吸道感染组患儿间差异均有统计学意义(P<0.05);呼吸道感染组工作特征曲线下面积比较EB-Ig M>EB-DNA>EB-Ig A>EB-Ig G;四项检测指标EB-Ig G、EB-DNA敏感性较好,EB-Ig M特异性较好。结论进行EB-Ig G、EB-Ig A、EB-Ig M三种抗体和EB-DNA检测对呼吸道感染性疾病有一定的诊断价值,不同年龄段的儿童由于免疫系统发育处于不同的发展阶段,对EB病毒的免疫应答存在差异。
Objective To investigate the diagnostic value of Epstein-Barr virus Ig G, Ig A, Ig M and EB-DNA in children with respiratory tract infectious diseases. Methods Serum Epstein-Barr virus (EBV) antibody was detected in 431 children with respiratory tract infection in Hunan Provincial People’s Hospital by enzyme-linked immunosorbent assay (ELISA), and throat swab or plasma EB-DNA was detected by PCR In the same period, 189 hospitalized children without respiratory tract infection served as control group. The positive rates of EB-DNA and EBVCA antibodies in children with respiratory tract infection at different ages were analyzed. The efficacy of each item in the diagnosis of respiratory tract infectious diseases was compared by ROC curve analysis. Results The positive rates of EB-DNA, Ig G and Ig M antibodies in respiratory tract infection group were significantly different (P <0.05). The area under the working characteristic curve of respiratory tract infection group was significantly lower than that of EB-Ig M EB-Ig> EB-Ig A> EB-Ig G. The four indexes of EB-Ig G and EB-DNA were better, and the specificity of EB-Ig M was better. Conclusions The detection of EB-Ig G, EB-Ig A and EB-Ig M antibodies and EB-DNA have certain diagnostic value for respiratory infectious diseases. Children of different ages are in different stages of development due to the development of immune system, There are differences in the immune response to Epstein-Barr virus.