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[目的]观察地塞米松对急性有机磷中毒小鼠敌敌畏模型的联合治疗作用。[方法]健康雄性昆明种小鼠36只,随机分为A、B、C 3组,各组小鼠均经口给予200 mg/kg敌敌畏染毒。A组为模型对照组,染毒后腹腔注射生理盐水;B组为常规治疗组,染毒后立即腹腔注射阿托品(15 mg/kg)和氯磷定(60 mg/kg);C组为地塞米松治疗组,在B组基础上联合应用地塞米松(5 mg/kg)。染毒后24 h内观察记录动物的中毒表现和存活情况。[结果]敌敌畏染毒后,A组小鼠症状出现早且重,10 min内动物全部死亡。B、C两组小鼠肌束震颤强度、翻正反射消失时间、存活时间、存活率、翘尾和流涎发生率等指标与A组比较,差异均有统计学意义(P=0.01、0.00、0.01、0.03、0.00、0.00,P<0.05);C组小鼠翻正反射消失时间和存活时间明显长于B组,差异有统计学意义(P<0.05)。[结论]地塞米松与阿托品和氯磷定联合应用,可有效控制动物的中毒症状,提高疗效。
[Objective] To observe the combined treatment of dexamethasone and dichlorvos in acute organophosphate poisoning mice. [Method] Thirty - six healthy male Kunming mice were randomly divided into A, B and C groups. All mice were orally given 200 mg / kg dichlorvos. Group A was model control group, intraperitoneal injection of normal saline was performed after exposure. Group B was treated routinely with atropine (15 mg / kg) and chlorpromazine (60 mg / kg) intraperitoneally. Group C Dexamethasone treatment group, combined with dexamethasone (5 mg / kg) on the basis of group B. Observed within 24 h after poisoning recorded the performance and survival of animals poisoning. [Result] After the dichlorvos was poisoned, the mice in group A appeared earlier and heavier, and all the animals died within 10 minutes. B group and C group had no significant difference in muscular bundle-tremor intensity, disappearance of righting reflex time, survival time, survival rate, tail-raising and salivation incidence compared with group A (P = 0.01, 0.01, 0.03, 0.00, 0.00, P <0.05). The disappearance time and survival time of group C were significantly longer than that of group B (P <0.05). [Conclusion] The combination of dexamethasone with atropine and chlorpromazine can effectively control the poisoning symptoms of animals and improve the curative effect.