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花生四烯酸代谢相关酶环加氧酶(COX)、脂氧合酶(LOX)和活化的胞外信号调节激酶(p-ERK1/2)等与乳腺癌发生发展具有密切关系.为了进一步阐明它们在乳腺癌转移中的作用及其分子机制,应用反转录PCR(RT-PCR)、免疫印迹和免疫组化等方法,分别检测了乳腺癌细胞系、乳腺癌组织、癌旁组织和转移淋巴结组织中COX-2、5-LOX、12R-LOX、cPLA2和p-ERK1/2的表达水平.结果显示,与对照组相比,在具有高转移潜能的乳腺癌LM-MCF-7细胞和MDA-MB-231细胞以及转移淋巴结组织中,COX-2、5-LOX、12R-LOX、cPLA2和p-ERK1/2均有较高水平表达.进而发现,p-ERK1/2的特异性抑制剂PD98059可拮抗LM-MCF-7细胞和MDA-MB-231细胞中COX-2、5-LOX、12R-LOX和cPLA2的高表达.上述结果表明,p-ERK1/2可以通过促进花生四烯酸代谢相关酶的表达促进乳腺癌细胞发生转移.
Cyclooxygenase (COX), lipoxygenase (LOX) and activated extracellular signal-regulated kinase (p-ERK1 / 2) in arachidonic acid metabolism are closely related to the development of breast cancer.In order to further elucidate Their roles in breast cancer metastasis and their molecular mechanisms were examined by reverse transcription-polymerase chain reaction (RT-PCR), Western blotting and immunohistochemistry. The effects of breast cancer cell lines, breast cancer tissues, adjacent tissues and metastasis The expression of COX-2, 5-LOX, 12R-LOX, cPLA2 and p-ERK1 / 2 in lymph node tissue were detected by RT-PCR.Results Compared with the control group, The levels of COX-2, 5-LOX, 12R-LOX, cPLA2 and p-ERK1 / 2 were significantly higher in MDA-MB-231 cells and metastatic lymph node tissues PD98059 antagonized the overexpression of COX-2,5-LOX, 12R-LOX and cPLA2 in LM-MCF-7 cells and MDA-MB-231 cells.The above results indicate that p-ERK1 / The expression of enzymes related to acid metabolism promotes the metastasis of breast cancer cells.