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从小麦根、茎、叶分离出21株溶磷微生物,筛选出溶磷能力最强的菌株JG-22,经形态特征、生理生化分析及16S rDNA序列分析,确定其为土壤芽孢杆菌(Brevibacillus agri strain).研究表明,JG-22对Ca3(PO4)2溶解能力最强;pH值对菌株JG-22溶磷效果影响最明显,在34～36℃和弱碱性环境下,溶磷效果较好;培养72 h后增加培养时间对有效磷的增量影响不大;Ca3(PO4)2含量在5 g/L以上时加大其含量对有效磷的增量几乎无影响.
Twenty-one strains of phosphate-solubilizing microorganisms were isolated from roots, stems and leaves of wheat, and the strain JG-22 with the highest ability to dissolve phosphorus was screened out. The morphological, physiological and biochemical analysis and 16S rDNA sequence analysis showed that the strain was Bacillus licheniformis (Brevibacillus agri The results showed that JG-22 had the strongest ability to dissolve Ca3 (PO4) 2 and the pH had the most obvious effect on the phosphate solubilization of strain JG-22. Under the conditions of 34-36 ℃ and weak alkaline environment, And the increase of culture time after 72 h had little effect on the increment of available phosphorus; if the content of Ca3 (PO4) 2 was above 5 g / L, its increase had little effect on the increase of available phosphorus.