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用ＭＴＴ法检测补骨脂素、γ干扰素及两者联合应用对人舌癌细胞系ＨＳＣ３的生长抑制作用，它们的半抑制浓度（ＬＣ５０）分别为２５１１９ｎｇ／ｍｌ和３７１５３５ｕ／ｍｌ。当γ干扰素浓度为１００ｕ／ｍｌ时与补骨脂素合用呈相加效应；浓度为１０００～１０００００ｕ／ｍｌ时合用呈协同效应，。补骨脂素、γ干扰素及两者联合应用对ＨＳＣ３细胞裸鼠移植瘤的抑瘤率分别为８３．１％、６７；６％和１０２．９％，两药合用呈协同效应；受试鼠体重减轻率分别为５％、１．２％和１．８％，对照组体重减轻１２．５％。结果提示联合用药具有明显的协同效应，而且有助于改善机体的全身情况。
The inhibitory effect of psoralen and interferon-γ on the growth of human tongue cancer cell line HSC3 was detected by MTT assay. Their half-inhibitory concentrations (LC50) were 25,119 ng / ml and 37,1535 u / ml, respectively. When γ interferon concentration of 100u / ml and psoralen combined effect was showed; when the concentration of 1000 ~ 100000u / ml synergistic effect when combined. The inhibitory rates of psoralen, interferon-γ and their combination on the xenografts of HSC3 nude mice were 83.1%, 67.6% and 102.9%, respectively. The combination of the two drugs showed a synergistic effect. Rat weight loss rates were 5%, 1.2% and 1.8%, respectively, in the control group 12.5% weight loss. The results suggest that combination therapy has obvious synergistic effect, and helps to improve the body's general condition.