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以毛新杨无性系TB0 1×毛白杨无性系LM5 0的回交子代 12 0株个体为作图群体 ,对控制叶片表型性状如叶长、叶宽、叶面积和叶柄长以及春季萌芽时间共 5个性状的数量性状位点 (quantitativetraitloci,QTLs)进行分析。运用AFLP标记技术结合拟测交作图策略构建了含有 393个AFLP标记的毛白杨及毛新杨的遗传连锁框架图。毛白杨遗传图上共含有 2 4 7个AFLP标记位点 ,连锁位点覆盖毛白杨基因组总长约 32 6 5 1cM ,而毛新杨遗传连锁图上共含有 14 6个标记位点 ,连锁位点覆盖毛新杨基因组总长约 1992cM ,这些连锁图的每一连锁群上含有的标记数为 4～ 30个。在此基础上 ,利用区间作图软件共检测到控制叶片表型性状的QTLs14个 ,位于 9个连锁群上 ;而对于春季萌芽时间共检测到 3个QTLs,分别位于 3个不同的连锁群上。在检测到的 17个QTLs中 ,每一QTL可解释表型变异的 7 6 %～ 15 8%。此外 ,发现控制叶长、叶宽和叶面积等相关性状的QTLs位于相同的基因组区域 ,这些QTLs主要位于毛白杨遗传连锁图的TLG2和TLG11以及毛新杨连锁图的TBLG1连锁群上。据控制叶片表型和春季萌芽时间的QTLs所处的基因组区域 ,可推测叶片表型和春季萌芽时间这两类性状是由各自相应的基因控制。
In this study, 120 individuals of backcross progenies of Mao xinyang clones TB0 1 × Populus tomentosa clones LM5 0 were used as the mapping population to control leaf phenotypic traits such as leaf length, leaf width, leaf area and petiole length, Time traits of quantitative trait loci (QTLs) were analyzed. AFLP markers were used to construct a genetic linkage map of 393 AFLP markers of Populus tomentosa and Populus tomentosa. A total of 247 AFLP loci were mapped on Populus tomentosa. The total length of the loci covered by Populus tomentosa was 32 6 5 1cM, whereas the Mao Xin Yang genetic linkage map contained 14 6 loci, The total length of the genome covered by Mao Yang Yang was about 1992cM, and the number of markers contained in each linkage group in these linkage maps was 4 to 30. Based on this, 14 QTLs controlling leaf phenotypic traits were detected by using interval mapping software, which were located on 9 linkage groups. Three QTLs were detected in spring germination time, which were located in 3 different linkage groups . Of the 17 QTLs detected, each QTL explained 76% -158% of phenotypic variation. In addition, it was found that the QTLs controlling leaf length, leaf width and leaf area were located in the same genomic region. These QTLs mainly located on the TLG2 and TLG11 genetic linkage map of Populus tomentosa and the TBLG1 linkage group of MaoXinYang linkage map. According to the genomic regions where QTLs controlling leaf phenotype and spring germination time are located, it is speculated that leaf phenotype and spring germination time are controlled by their respective genes.