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以黑龙江省大豆重茬 5年与正茬土壤和根系为主要研究对象 ,采用高效液相色谱法 ,研究土壤和根系浸提液中的酚酸物质的含量及其生物学效应 .结果表明 ,重茬土壤中对羟基苯甲酸和香草酸的含量 (1mol·L-1NaOH提取 )大于正茬土壤 ,且差异达到极显著水平 ,香草醛含量差异不显著 ;重茬大豆根系水提液中对羟基苯甲酸、香草酸、阿魏酸、香草醛、香豆素含量均高于正茬 .大豆连作条件下土壤多酚氧化酶活性高于正茬土壤 .重茬大豆根系水提液及在水培条件下外加对羟基苯甲酸对大豆幼苗生长发育有一定的抑制作用 ;酚酸物质加入土壤 1周后 ,对羟基苯甲酸、香草酸、香草醛、阿魏酸、苯甲酸、香豆素残留率分别为 10 .4%、15 .3 %、4.1%、2 .3 %、5 .0 %、17.5 % ;且外加酚酸浓度与土壤中真菌数量呈极显著指数相关 .
In this study, the soil and root system of soybean under continuous cropping of soybean in Heilongjiang Province for five years were selected as the main research object, and the contents of phenolic acids and their biological effects in soil and root extracts were studied by HPLC. The results showed that soil The contents of p-hydroxybenzoic acid and vanillic acid (1mol·L-1NaOH) were higher than that of the positive soil, and the difference was significant. The difference of vanillin content was not significant. The content of p-hydroxybenzoic acid, vanillic acid , The contents of ferulic acid, vanillin and coumarin were all higher than that of the positive crop.The soil polyphenol oxidase activity in soybean continuous cropping was higher than that of the positive soil, Formic acid had certain inhibitory effects on the growth and development of soybean seedlings. The residual rates of p-hydroxybenzoic acid, vanillic acid, vanillin, ferulic acid, benzoic acid and coumarin were 1 .4% , 15.3%, 4.1%, 2.3%, 5.0% and 17.5%, respectively. Moreover, the concentration of phenolic acids was significantly and exponentially correlated with the number of fungi in the soil.