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目的:比较植块培养法与胰蛋白酶消化法原代培养口腔鳞状细胞癌组织,并探讨培养成功的临床影响因素。方法:分别用植块培养法和胰蛋白酶消化法,对48例人口腔鳞状细胞癌组织进行体外培养,观察癌细胞生长情况。结果:①48例样本中24例培养成功,细胞生长率50%。②植块培养法优于胰蛋白酶消化培养法,植块培养法细胞生长率38%,胰蛋白酶消化培养法细胞生长率13%(p<0.01)。③高分化鳞癌生长率为38%,中/低分化鳞癌生长率为75%(p<0.05)。结论:优化的植块培养法比单纯消化培养法能尽快获得口腔癌的原代细胞系,种子细胞应该尽量选用分化程度差的标本。
OBJECTIVE: To compare the primary culture of oral squamous cell carcinoma with explant culture and trypsin digestion, and to explore the clinical influencing factors of successful culture. Methods: Forty-eight human oral squamous cell carcinoma tissues were cultured in vitro by means of explant culture and trypsin digestion, and the growth of cancer cells was observed. Results: ①48 cases of 48 cases were successfully cultured, the cell growth rate of 50%. ② The method of explant culture was better than that of trypsin digestion. The cell growth rate was 38% in explant culture and 13% in trypsin digestion (p <0.01). ③ well-differentiated squamous cell carcinoma growth rate of 38%, moderate / poorly differentiated squamous cell carcinoma growth rate of 75% (p <0.05). Conclusion: The optimized culture method can obtain the primary cell line of oral cancer as soon as possible by simple digestion culture method, and the seed cells should try their best to select the samples with poor differentiation degree.