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目的对南海海洋沉积物中分离的海洋放线菌进行体外抗肿瘤细胞和抗菌模型筛选,选取生物活性强的海洋链霉菌菌株Streptomyces sp.SCSIO1666进行发酵条件优化和活性产物的分离及结构鉴定。方法用针对6种NCI肿瘤细胞株A549、DU145、H1299、HCT15、HEP3B和SF268的体外抗肿瘤模型及其抑菌模型对提取物进行筛选,进一步优化发酵条件,采用HPLC-UV和枯草芽孢杆菌(Bacillussubtilis)抑菌活性追踪,利用有机溶剂萃取、正相硅胶和反相硅胶等各种色谱层析分离,通过理化性质和波谱数据分析进行结构鉴定。结果与结论从一株海洋链霉菌菌株Streptomyces sp.SCSIO 1666发酵产物中分离得到替达霉素A和B(TirandamycinsA and B),替达霉素A和B的生产对海盐的依赖程度较高,在不加盐的条件下产量极低,加3%粗海盐时,其发酵产量提高250倍以上,说明盐胁迫是海洋链霉菌菌株SCSIO1666产生活性物质的重要条件。替达霉素B是终产物,加入2%大孔吸附树脂发酵时,替达霉素A的产量得到明显提高。
OBJECTIVE To screen the marine actinomycetes isolated from marine sediments in the South China Sea for screening anti-tumor cells and antibacterial models in vitro and to select the biologically active Streptomyces sp. SCSIO1666 strain for the optimization of fermentation conditions and the isolation and identification of the active products. Methods The extracts were screened by in vitro anti-tumor model and antibacterial model against six kinds of NCI tumor cell lines A549, DU145, H1299, HCT15, HEP3B and SF268. The fermentation conditions were further optimized by HPLC-UV and Bacillus subtilis Bacillussubtilis), and its structure was identified by physico-chemical properties and spectroscopic data analysis using various organic solvents, normal phase silica gel and reverse phase silica gel. RESULTS AND CONCLUSION Termitomycin A and B (Tirandamycins A and B) were isolated from the fermentation product of Streptomyces sp. SCSIO 1666 strain. The production of teichomycin A and B was highly dependent on sea salt, Under the condition of no salt, the yield was very low. When 3% crude sea salt was added, its fermentation yield increased more than 250 times, indicating that salt stress is an important condition for the production of active substance by the marine streptomyces strain SCSIO1666. Ticarubicin B is the final product, adding 2% macroporous resin fermentation, the yield of tamycin A was significantly improved.