目的研究肿瘤坏死因子（ＴＮＦα）对溶血卵磷脂（ＬＰＣ）引起脑微血管平滑肌细胞（ＳＭＣ）增殖的影响及维生素Ｅ（ＶｉｔＥ），普罗布考（ＰＲＢ）的保护作用。方法用结晶紫染色测定密度（ＯＤ）值的方法观察ＴＮＦα对ＬＰＣ引起牛脑微血管平滑肌细胞（ＢＣＭＳＭＣ）增殖作用的影响。结果ＴＮＦα在２５×１０４～１×１０５Ｕ·Ｌ－１范围内能协同ＬＰＣ引起ＢＣＭＳＭＣ增殖，８ｈ时增殖率就明显增加，１６ｈ增殖率达峰值（６７２％）。ＶｉｔＥ２０，５０，１００μｍｏｌ·Ｌ－１，ＰＲＢ１０，２０，５０μｍｏｌ·Ｌ－１呈剂量依赖性地显著降低其增殖率，其最大抑制率分别为７４２％和４９７％。结论ＴＮＦα对ＬＰＣ引起的ＢＣＭＳＭＣ增殖有协同作用，药物ＶｉｔＥ，ＰＲＢ可拮抗其增殖作用。 Objective To study the effect of tumor necrosis factor α (TNFα) on the proliferation of cerebral microvascular smooth muscle cells (SMC) induced by lysophosphatidylcholine (LPC) and the protective effect of vitamin E and probucol (PRB). Methods The method of crystal violet staining determination of density (OD) was used to observe the effects of TNFα on the proliferation of bovine cerebral microvascular smooth muscle cells (BCMSMC) induced by LPC. Results TNF  α could promote the proliferation of BCMSMC with LPC in the range of 2.5 × 104-1 × 105U · L-1. The proliferation rate increased significantly at 8h and reached the peak at 16h (672%). VitE20,50,100μmol·L-1, PRB10,20,50μmol·L-1 significantly reduced the proliferation rate in a dose-dependent manner, with the maximum inhibition rates of 742% and 497%, respectively. Conclusion TNF @ LPC-induced proliferation of BCMSMC have a synergistic effect, the drug VitE, PRB can antagonize its proliferation.