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应用体内试验将分离制备的日本血吸虫成虫分泌排泄物和浸出液注入健康豚鼠背部皮内,1~48h取其组织观察嗜酸酸性粒细胞和中性粒细胞数量。在注射后2~4h,注射部位可见少量嗜性粒细胞,8h细胞数量达高峰,然后减少,24h后只见少量嗜酸性粒细胞。在注射后1h,注射部位中性粒细胞增多,4h细胞数量最多,然后逐渐减少,48h降至最低。结果证明日本血吸虫成虫对嗜酸性粒细胞和中性粒细胞有明显的趋化作用。
In vivo tests were conducted to separate and prepare excreted secretions and leachate from adult Schistosoma japonicum, which were prepared separately, into the dorsal skin of healthy guinea pigs. The tissues were observed for the number of eosinophils and neutrophils from 1 to 48 hours. At 2 ~ 4h after injection, a small amount of tropheocytes were observed at the injection site, reached the peak at 8h, and then decreased. A small amount of eosinophils were seen after 24h. At 1 hour after injection, the number of neutrophils in the injection site increased and reached the peak at 4 hours, then decreased gradually and decreased to the lowest level at 48 hours. The results show that adult Schistosoma japonicum has obvious chemotactic effect on eosinophils and neutrophils.