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目的分析戊型肝炎病毒(HEV)ORF2N-端和C-端多肽的抗原性。方法以纯化的4型HEVORF2N-端1～124aa多肽pS4-1和4型HEVORF2C-端385～674aa多肽pS4-6分别包被微孔板,采用间接酶联免疫法(EIA)检测感染HEV的猴系列血清中的抗HEVIgG抗体,分析两种多肽的抗原性,并与进口试剂的检测结果进行比较。结果在感染HEV的猴系列血清中,针对HEVORF2N-端抗原的抗体产生时间早,持续时间短,其峰值与转氨酶(ALT)的峰值几乎重叠;针对HEVORF2C-端抗原的抗体在HEV感染早期产生的滴度较低,但逐渐升高,在14周的观察期内,抗体滴度几乎无下降趋势。用两种多肽检测HEVIgG抗体的灵敏度均高于进口试剂。结论两种多肽均具有较好的抗原性,但在HEV感染的不同时期,其反应性不同。
Objective To analyze the antigenicity of ORF2N-terminal and C-terminal polypeptides of hepatitis E virus (HEV). Methods Purified HEVORF2 N-terminal 1 ~ 124aa polypeptide pS4-1 and HEVORF2 C-terminal 385 ~ 674aa polypeptide pS4-6 were respectively coated on microplate and HEV-infected monkeys were detected by indirect enzyme-linked immunosorbent assay (EIA) Serum anti-HEVIgG antibodies were analyzed for antigenicity of the two polypeptides and compared with the test results of imported reagents. Results The HEVORF2N-terminal antigens produced by the HEVORF2N-terminal antigens in the sera of HEVOF2 infected early-onset and short-duration were almost overlapped with the peaks of the aminotransferase (ALT). The antibodies directed against the HEVORF2 C-terminal antigen were detected in the early stages of HEV infection The titers were lower but gradually increased, and there was almost no decrease in antibody titers during the 14 week observation period. The sensitivity of HEVIgG antibodies detected by both peptides was higher than that of imported reagents. Conclusion Both peptides have good antigenicity, but their reactivity is different at different stages of HEV infection.