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本研究以不同含盐量的滨海盐土、内陆盐碱土和中等肥力非盐碱土壤为实验对象,探讨花生种子在吸水膨胀与萌发过程中,不同类型盐碱土对种子际土壤微生物多样性变化的影响。本实验对不同土壤样品中细菌的16SrRNA基因的V3-V4区进行PCR扩增;利用高通量测序的方法对12份V3-V4高变区PCR产物进行测序,并对测序数据进行生物信息学分析。结果显示:(1)东营青坨滨海盐土种子际土壤细菌群落多样性大于聊城高唐内陆盐碱土花生种子际土壤细菌群落多样性。(2)不同类型土壤样本微生物群落结构在纲水平存在明显差异。4种土壤中的种子际细菌共分属于6个菌纲,分别为Proteobacteria、Actinobacteria、Actinobacteria、Bacteroidetes、Acidobacteria和Firmicutes菌纲,并均以Proteobacteria和Actinobacteria菌纲为主要菌纲。全样本菌落结构分析结果表明,4种类型土壤中不同吸胀时间内种子际微生物菌落在门、属水平上的类型和丰度差异最为显著(p<0.05)。(3)beta多样性分析和各样本遗传距离(phylogenetic distances)聚类树图分析表明,4个土壤类型的12个土壤样本种子际土壤中微生物群落均可聚为2大类。上述研究结果为盐碱地花生保全苗、促早发提供了重要的理论依据。
In this study, coastal saline soil, inland saline soil and mid-fertility non-saline soil with different salt content were used as experimental subjects to study the effects of different types of saline-alkali soil on the changes of soil microbial diversity in the process of water absorption expansion and germination of peanut seeds influences. In this study, the V3-V4 region of 16SrRNA gene of bacteria in different soil samples was amplified by PCR. 12 V3-V4 hypervariable region PCR products were sequenced by high-throughput sequencing and the bioinformatics analysis. The results showed that: (1) The diversity of soil bacterial community in coastal saline soil of Dongying was greater than that in saline soil of Liaocheng Gaotang. (2) There are obvious differences in the community level of microbial community structure in different types of soil samples. The four kinds of soil seed bacteria were divided into 6 groups, which were Proteobacteria, Actinobacteria, Actinobacteria, Bacteroidetes, Acidobacteria and Firmicutes, and Proteobacteria and Actinobacteria were the main fungi. The results of colony structure analysis of the whole sample showed that there were significant differences (P <0.05) in the types and abundances of the interspecific microbial colonies at the door and genus in different types of soils in four types of soil. (3) The results of beta diversity analysis and phylogenetic distances clustering tree analysis showed that the microbial community in 12 soils of 4 soil types could all be grouped into 2 groups. The above results provide important theoretical basis for preserving seedlings of peanut in saline-alkali soil and promoting early development.